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Making statistics intuitive

Hypothesis Testing: Uses, Steps & Example

By Jim Frost 4 Comments

What is Hypothesis Testing?

Hypothesis testing in statistics uses sample data to infer the properties of a whole population . These tests determine whether a random sample provides sufficient evidence to conclude an effect or relationship exists in the population. Researchers use them to help separate genuine population-level effects from false effects that random chance can create in samples. These methods are also known as significance testing.

For example, researchers are testing a new medication to see if it lowers blood pressure. They compare a group taking the drug to a control group taking a placebo. If their hypothesis test results are statistically significant, the medication’s effect of lowering blood pressure likely exists in the broader population, not just the sample studied.

Using Hypothesis Tests

A hypothesis test evaluates two mutually exclusive statements about a population to determine which statement the sample data best supports. These two statements are called the null hypothesis and the alternative hypothesis . The following are typical examples:

• Null Hypothesis : The effect does not exist in the population.
• Alternative Hypothesis : The effect does exist in the population.

Hypothesis testing accounts for the inherent uncertainty of using a sample to draw conclusions about a population, which reduces the chances of false discoveries. These procedures determine whether the sample data are sufficiently inconsistent with the null hypothesis that you can reject it. If you can reject the null, your data favor the alternative statement that an effect exists in the population.

Statistical significance in hypothesis testing indicates that an effect you see in sample data also likely exists in the population after accounting for random sampling error , variability, and sample size. Your results are statistically significant when the p-value is less than your significance level or, equivalently, when your confidence interval excludes the null hypothesis value.

Conversely, non-significant results indicate that despite an apparent sample effect, you can’t be sure it exists in the population. It could be chance variation in the sample and not a genuine effect.

Learn more about Failing to Reject the Null .

5 Steps of Significance Testing

Hypothesis testing involves five key steps, each critical to validating a research hypothesis using statistical methods:

• Formulate the Hypotheses : Write your research hypotheses as a null hypothesis (H 0 ) and an alternative hypothesis (H A ).
• Data Collection : Gather data specifically aimed at testing the hypothesis.
• Conduct A Test : Use a suitable statistical test to analyze your data.
• Make a Decision : Based on the statistical test results, decide whether to reject the null hypothesis or fail to reject it.
• Report the Results : Summarize and present the outcomes in your report’s results and discussion sections.

While the specifics of these steps can vary depending on the research context and the data type, the fundamental process of hypothesis testing remains consistent across different studies.

Let’s work through these steps in an example!

Hypothesis Testing Example

Researchers want to determine if a new educational program improves student performance on standardized tests. They randomly assign 30 students to a control group , which follows the standard curriculum, and another 30 students to a treatment group, which participates in the new educational program. After a semester, they compare the test scores of both groups.

Download the CSV data file to perform the hypothesis testing yourself: Hypothesis_Testing .

The researchers write their hypotheses. These statements apply to the population, so they use the mu (μ) symbol for the population mean parameter .

• Null Hypothesis (H 0 ) : The population means of the test scores for the two groups are equal (μ 1 = μ 2 ).
• Alternative Hypothesis (H A ) : The population means of the test scores for the two groups are unequal (μ 1 ≠ μ 2 ).

Choosing the correct hypothesis test depends on attributes such as data type and number of groups. Because they’re using continuous data and comparing two means, the researchers use a 2-sample t-test .

Here are the results.

The treatment group’s mean is 58.70, compared to the control group’s mean of 48.12. The mean difference is 10.67 points. Use the test’s p-value and significance level to determine whether this difference is likely a product of random fluctuation in the sample or a genuine population effect.

Because the p-value (0.000) is less than the standard significance level of 0.05, the results are statistically significant, and we can reject the null hypothesis. The sample data provides sufficient evidence to conclude that the new program’s effect exists in the population.

Limitations

Hypothesis testing improves your effectiveness in making data-driven decisions. However, it is not 100% accurate because random samples occasionally produce fluky results. Hypothesis tests have two types of errors, both relating to drawing incorrect conclusions.

• Type I error: The test rejects a true null hypothesis—a false positive.
• Type II error: The test fails to reject a false null hypothesis—a false negative.

Learn more about Type I and Type II Errors .

Our exploration of hypothesis testing using a practical example of an educational program reveals its powerful ability to guide decisions based on statistical evidence. Whether you’re a student, researcher, or professional, understanding and applying these procedures can open new doors to discovering insights and making informed decisions. Let this tool empower your analytical endeavors as you navigate through the vast seas of data.

Learn more about the Hypothesis Tests for Various Data Types .

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Reader Interactions

June 10, 2024 at 10:51 am

Thank you, Jim, for another helpful article; timely too since I have started reading your new book on hypothesis testing and, now that we are at the end of the school year, my district is asking me to perform a number of evaluations on instructional programs. This is where my question/concern comes in. You mention that hypothesis testing is all about testing samples. However, I use all the students in my district when I make these comparisons. Since I am using the entire “population” in my evaluations (I don’t select a sample of third grade students, for example, but I use all 700 third graders), am I somehow misusing the tests? Or can I rest assured that my district’s student population is only a sample of the universal population of students?

June 10, 2024 at 1:50 pm

I hope you are finding the book helpful!

Yes, the purpose of hypothesis testing is to infer the properties of a population while accounting for random sampling error.

In your case, it comes down to how you want to use the results. Who do you want the results to apply to?

If you’re summarizing the sample, looking for trends and patterns, or evaluating those students and don’t plan to apply those results to other students, you don’t need hypothesis testing because there is no sampling error. They are the population and you can just use descriptive statistics. In this case, you’d only need to focus on the practical significance of the effect sizes.

On the other hand, if you want to apply the results from this group to other students, you’ll need hypothesis testing. However, there is the complicating issue of what population your sample of students represent. I’m sure your district has its own unique characteristics, demographics, etc. Your district’s students probably don’t adequately represent a universal population. At the very least, you’d need to recognize any special attributes of your district and how they could bias the results when trying to apply them outside the district. Or they might apply to similar districts in your region.

However, I’d imagine your 3rd graders probably adequately represent future classes of 3rd graders in your district. You need to be alert to changing demographics. At least in the short run I’d imagine they’d be representative of future classes.

Think about how these results will be used. Do they just apply to the students you measured? Then you don’t need hypothesis tests. However, if the results are being used to infer things about other students outside of the sample, you’ll need hypothesis testing along with considering how well your students represent the other students and how they differ.

I hope that helps!

June 10, 2024 at 3:21 pm

Thank you so much, Jim, for the suggestions in terms of what I need to think about and consider! You are always so clear in your explanations!!!!

June 10, 2024 at 3:22 pm

You’re very welcome! Best of luck with your evaluations!

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• How it works

Hypothesis Testing – A Complete Guide with Examples

Published by Alvin Nicolas at August 14th, 2021 , Revised On October 26, 2023

In statistics, hypothesis testing is a critical tool. It allows us to make informed decisions about populations based on sample data. Whether you are a researcher trying to prove a scientific point, a marketer analysing A/B test results, or a manufacturer ensuring quality control, hypothesis testing plays a pivotal role. This guide aims to introduce you to the concept and walk you through real-world examples.

What is a Hypothesis and a Hypothesis Testing?

A hypothesis is considered a belief or assumption that has to be accepted, rejected, proved or disproved. In contrast, a research hypothesis is a research question for a researcher that has to be proven correct or incorrect through investigation.

What is Hypothesis Testing?

Hypothesis testing  is a scientific method used for making a decision and drawing conclusions by using a statistical approach. It is used to suggest new ideas by testing theories to know whether or not the sample data supports research. A research hypothesis is a predictive statement that has to be tested using scientific methods that join an independent variable to a dependent variable.  

Example: The academic performance of student A is better than student B

Characteristics of the Hypothesis to be Tested

A hypothesis should be:

• Clear and precise
• Capable of being tested
• Able to relate to a variable
• Stated in simple terms
• Consistent with known facts
• Limited in scope and specific
• Tested in a limited timeframe
• Explain the facts in detail

What is a Null Hypothesis and Alternative Hypothesis?

A  null hypothesis  is a hypothesis when there is no significant relationship between the dependent and the participants’ independent  variables . 

In simple words, it’s a hypothesis that has been put forth but hasn’t been proved as yet. A researcher aims to disprove the theory. The abbreviation “Ho” is used to denote a null hypothesis.

If you want to compare two methods and assume that both methods are equally good, this assumption is considered the null hypothesis.

Example: In an automobile trial, you feel that the new vehicle’s mileage is similar to the previous model of the car, on average. You can write it as: Ho: there is no difference between the mileage of both vehicles. If your findings don’t support your hypothesis and you get opposite results, this outcome will be considered an alternative hypothesis.

If you assume that one method is better than another method, then it’s considered an alternative hypothesis. The alternative hypothesis is the theory that a researcher seeks to prove and is typically denoted by H1 or HA.

If you support a null hypothesis, it means you’re not supporting the alternative hypothesis. Similarly, if you reject a null hypothesis, it means you are recommending the alternative hypothesis.

Example: In an automobile trial, you feel that the new vehicle’s mileage is better than the previous model of the vehicle. You can write it as; Ha: the two vehicles have different mileage. On average/ the fuel consumption of the new vehicle model is better than the previous model.

If a null hypothesis is rejected during the hypothesis test, even if it’s true, then it is considered as a type-I error. On the other hand, if you don’t dismiss a hypothesis, even if it’s false because you could not identify its falseness, it’s considered a type-II error.

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How to Conduct Hypothesis Testing?

Here is a step-by-step guide on how to conduct hypothesis testing.

Step 1: State the Null and Alternative Hypothesis

Once you develop a research hypothesis, it’s important to state it is as a Null hypothesis (Ho) and an Alternative hypothesis (Ha) to test it statistically.

A null hypothesis is a preferred choice as it provides the opportunity to test the theory. In contrast, you can accept the alternative hypothesis when the null hypothesis has been rejected.

Example: You want to identify a relationship between obesity of men and women and the modern living style. You develop a hypothesis that women, on average, gain weight quickly compared to men. Then you write it as: Ho: Women, on average, don’t gain weight quickly compared to men. Ha: Women, on average, gain weight quickly compared to men.

Step 2: Data Collection

Hypothesis testing follows the statistical method, and statistics are all about data. It’s challenging to gather complete information about a specific population you want to study. You need to  gather the data  obtained through a large number of samples from a specific population. 

Example: Suppose you want to test the difference in the rate of obesity between men and women. You should include an equal number of men and women in your sample. Then investigate various aspects such as their lifestyle, eating patterns and profession, and any other variables that may influence average weight. You should also determine your study’s scope, whether it applies to a specific group of population or worldwide population. You can use available information from various places, countries, and regions.

Step 3: Select Appropriate Statistical Test

There are many  types of statistical tests , but we discuss the most two common types below, such as One-sided and two-sided tests.

Note: Your choice of the type of test depends on the purpose of your study 

One-sided Test

In the one-sided test, the values of rejecting a null hypothesis are located in one tail of the probability distribution. The set of values is less or higher than the critical value of the test. It is also called a one-tailed test of significance.

Example: If you want to test that all mangoes in a basket are ripe. You can write it as: Ho: All mangoes in the basket, on average, are ripe. If you find all ripe mangoes in the basket, the null hypothesis you developed will be true.

Two-sided Test

In the two-sided test, the values of rejecting a null hypothesis are located on both tails of the probability distribution. The set of values is less or higher than the first critical value of the test and higher than the second critical value test. It is also called a two-tailed test of significance. 

Example: Nothing can be explicitly said whether all mangoes are ripe in the basket. If you reject the null hypothesis (Ho: All mangoes in the basket, on average, are ripe), then it means all mangoes in the basket are not likely to be ripe. A few mangoes could be raw as well.

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Step 4: Select the Level of Significance

When you reject a null hypothesis, even if it’s true during a statistical hypothesis, it is considered the  significance level . It is the probability of a type one error. The significance should be as minimum as possible to avoid the type-I error, which is considered severe and should be avoided. 

If the significance level is minimum, then it prevents the researchers from false claims. 

The significance level is denoted by  P,  and it has given the value of 0.05 (P=0.05)

If the P-Value is less than 0.05, then the difference will be significant. If the P-value is higher than 0.05, then the difference is non-significant.

Example: Suppose you apply a one-sided test to test whether women gain weight quickly compared to men. You get to know about the average weight between men and women and the factors promoting weight gain.

Step 5: Find out Whether the Null Hypothesis is Rejected or Supported

After conducting a statistical test, you should identify whether your null hypothesis is rejected or accepted based on the test results. It would help if you observed the P-value for this.

Example: If you find the P-value of your test is less than 0.5/5%, then you need to reject your null hypothesis (Ho: Women, on average, don’t gain weight quickly compared to men). On the other hand, if a null hypothesis is rejected, then it means the alternative hypothesis might be true (Ha: Women, on average, gain weight quickly compared to men. If you find your test’s P-value is above 0.5/5%, then it means your null hypothesis is true.

Step 6: Present the Outcomes of your Study

The final step is to present the  outcomes of your study . You need to ensure whether you have met the objectives of your research or not. 

In the discussion section and  conclusion , you can present your findings by using supporting evidence and conclude whether your null hypothesis was rejected or supported.

In the result section, you can summarise your study’s outcomes, including the average difference and P-value of the two groups.

If we talk about the findings, our study your results will be as follows:

Example: In the study of identifying whether women gain weight quickly compared to men, we found the P-value is less than 0.5. Hence, we can reject the null hypothesis (Ho: Women, on average, don’t gain weight quickly than men) and conclude that women may likely gain weight quickly than men.

Did you know in your academic paper you should not mention whether you have accepted or rejected the null hypothesis? 

Always remember that you either conclude to reject Ho in favor of Haor   do not reject Ho . It would help if you never rejected  Ha  or even  accept Ha .

Suppose your null hypothesis is rejected in the hypothesis testing. If you conclude  reject Ho in favor of Haor   do not reject Ho,  then it doesn’t mean that the null hypothesis is true. It only means that there is a lack of evidence against Ho in favour of Ha. If your null hypothesis is not true, then the alternative hypothesis is likely to be true.

Example: We found that the P-value is less than 0.5. Hence, we can conclude reject Ho in favour of Ha (Ho: Women, on average, don’t gain weight quickly than men) reject Ho in favour of Ha. However, rejected in favour of Ha means (Ha: women may likely to gain weight quickly than men)

Frequently Asked Questions

What are the 3 types of hypothesis test.

The 3 types of hypothesis tests are:

• One-Sample Test : Compare sample data to a known population value.
• Two-Sample Test : Compare means between two sample groups.
• ANOVA : Analyze variance among multiple groups to determine significant differences.

What is a hypothesis?

A hypothesis is a proposed explanation or prediction about a phenomenon, often based on observations. It serves as a starting point for research or experimentation, providing a testable statement that can either be supported or refuted through data and analysis. In essence, it’s an educated guess that drives scientific inquiry.

What are null hypothesis?

A null hypothesis (often denoted as H0) suggests that there is no effect or difference in a study or experiment. It represents a default position or status quo. Statistical tests evaluate data to determine if there’s enough evidence to reject this null hypothesis.

What is the probability value?

The probability value, or p-value, is a measure used in statistics to determine the significance of an observed effect. It indicates the probability of obtaining the observed results, or more extreme, if the null hypothesis were true. A small p-value (typically <0.05) suggests evidence against the null hypothesis, warranting its rejection.

What is p value?

The p-value is a fundamental concept in statistical hypothesis testing. It represents the probability of observing a test statistic as extreme, or more so, than the one calculated from sample data, assuming the null hypothesis is true. A low p-value suggests evidence against the null, possibly justifying its rejection.

What is a t test?

A t-test is a statistical test used to compare the means of two groups. It determines if observed differences between the groups are statistically significant or if they likely occurred by chance. Commonly applied in research, there are different t-tests, including independent, paired, and one-sample, tailored to various data scenarios.

When to reject null hypothesis?

Reject the null hypothesis when the test statistic falls into a predefined rejection region or when the p-value is less than the chosen significance level (commonly 0.05). This suggests that the observed data is unlikely under the null hypothesis, indicating evidence for the alternative hypothesis. Always consider the study’s context.

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The Craft of Writing a Strong Hypothesis

Table of Contents

Writing a hypothesis is one of the essential elements of a scientific research paper. It needs to be to the point, clearly communicating what your research is trying to accomplish. A blurry, drawn-out, or complexly-structured hypothesis can confuse your readers. Or worse, the editor and peer reviewers.

A captivating hypothesis is not too intricate. This blog will take you through the process so that, by the end of it, you have a better idea of how to convey your research paper's intent in just one sentence.

What is a Hypothesis?

The first step in your scientific endeavor, a hypothesis, is a strong, concise statement that forms the basis of your research. It is not the same as a thesis statement , which is a brief summary of your research paper .

The sole purpose of a hypothesis is to predict your paper's findings, data, and conclusion. It comes from a place of curiosity and intuition . When you write a hypothesis, you're essentially making an educated guess based on scientific prejudices and evidence, which is further proven or disproven through the scientific method.

The reason for undertaking research is to observe a specific phenomenon. A hypothesis, therefore, lays out what the said phenomenon is. And it does so through two variables, an independent and dependent variable.

The independent variable is the cause behind the observation, while the dependent variable is the effect of the cause. A good example of this is “mixing red and blue forms purple.” In this hypothesis, mixing red and blue is the independent variable as you're combining the two colors at your own will. The formation of purple is the dependent variable as, in this case, it is conditional to the independent variable.

Different Types of Hypotheses‌

Types of hypotheses

Some would stand by the notion that there are only two types of hypotheses: a Null hypothesis and an Alternative hypothesis. While that may have some truth to it, it would be better to fully distinguish the most common forms as these terms come up so often, which might leave you out of context.

Apart from Null and Alternative, there are Complex, Simple, Directional, Non-Directional, Statistical, and Associative and casual hypotheses. They don't necessarily have to be exclusive, as one hypothesis can tick many boxes, but knowing the distinctions between them will make it easier for you to construct your own.

1. Null hypothesis

A null hypothesis proposes no relationship between two variables. Denoted by H 0 , it is a negative statement like “Attending physiotherapy sessions does not affect athletes' on-field performance.” Here, the author claims physiotherapy sessions have no effect on on-field performances. Even if there is, it's only a coincidence.

2. Alternative hypothesis

Considered to be the opposite of a null hypothesis, an alternative hypothesis is donated as H1 or Ha. It explicitly states that the dependent variable affects the independent variable. A good  alternative hypothesis example is “Attending physiotherapy sessions improves athletes' on-field performance.” or “Water evaporates at 100 °C. ” The alternative hypothesis further branches into directional and non-directional.

• Directional hypothesis: A hypothesis that states the result would be either positive or negative is called directional hypothesis. It accompanies H1 with either the ‘<' or ‘>' sign.
• Non-directional hypothesis: A non-directional hypothesis only claims an effect on the dependent variable. It does not clarify whether the result would be positive or negative. The sign for a non-directional hypothesis is ‘≠.'

3. Simple hypothesis

A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, “Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking.

4. Complex hypothesis

In contrast to a simple hypothesis, a complex hypothesis implies the relationship between multiple independent and dependent variables. For instance, “Individuals who eat more fruits tend to have higher immunity, lesser cholesterol, and high metabolism.” The independent variable is eating more fruits, while the dependent variables are higher immunity, lesser cholesterol, and high metabolism.

5. Associative and casual hypothesis

Associative and casual hypotheses don't exhibit how many variables there will be. They define the relationship between the variables. In an associative hypothesis, changing any one variable, dependent or independent, affects others. In a casual hypothesis, the independent variable directly affects the dependent.

6. Empirical hypothesis

Also referred to as the working hypothesis, an empirical hypothesis claims a theory's validation via experiments and observation. This way, the statement appears justifiable and different from a wild guess.

Say, the hypothesis is “Women who take iron tablets face a lesser risk of anemia than those who take vitamin B12.” This is an example of an empirical hypothesis where the researcher  the statement after assessing a group of women who take iron tablets and charting the findings.

7. Statistical hypothesis

The point of a statistical hypothesis is to test an already existing hypothesis by studying a population sample. Hypothesis like “44% of the Indian population belong in the age group of 22-27.” leverage evidence to prove or disprove a particular statement.

Characteristics of a Good Hypothesis

Writing a hypothesis is essential as it can make or break your research for you. That includes your chances of getting published in a journal. So when you're designing one, keep an eye out for these pointers:

• A research hypothesis has to be simple yet clear to look justifiable enough.
• It has to be testable — your research would be rendered pointless if too far-fetched into reality or limited by technology.
• It has to be precise about the results —what you are trying to do and achieve through it should come out in your hypothesis.
• A research hypothesis should be self-explanatory, leaving no doubt in the reader's mind.
• If you are developing a relational hypothesis, you need to include the variables and establish an appropriate relationship among them.
• A hypothesis must keep and reflect the scope for further investigations and experiments.

Separating a Hypothesis from a Prediction

Outside of academia, hypothesis and prediction are often used interchangeably. In research writing, this is not only confusing but also incorrect. And although a hypothesis and prediction are guesses at their core, there are many differences between them.

A hypothesis is an educated guess or even a testable prediction validated through research. It aims to analyze the gathered evidence and facts to define a relationship between variables and put forth a logical explanation behind the nature of events.

Predictions are assumptions or expected outcomes made without any backing evidence. They are more fictionally inclined regardless of where they originate from.

For this reason, a hypothesis holds much more weight than a prediction. It sticks to the scientific method rather than pure guesswork. "Planets revolve around the Sun." is an example of a hypothesis as it is previous knowledge and observed trends. Additionally, we can test it through the scientific method.

Whereas "COVID-19 will be eradicated by 2030." is a prediction. Even though it results from past trends, we can't prove or disprove it. So, the only way this gets validated is to wait and watch if COVID-19 cases end by 2030.

Finally, How to Write a Hypothesis

Quick tips on writing a hypothesis

1.  Be clear about your research question

A hypothesis should instantly address the research question or the problem statement. To do so, you need to ask a question. Understand the constraints of your undertaken research topic and then formulate a simple and topic-centric problem. Only after that can you develop a hypothesis and further test for evidence.

2. Carry out a recce

Once you have your research's foundation laid out, it would be best to conduct preliminary research. Go through previous theories, academic papers, data, and experiments before you start curating your research hypothesis. It will give you an idea of your hypothesis's viability or originality.

Making use of references from relevant research papers helps draft a good research hypothesis. SciSpace Discover offers a repository of over 270 million research papers to browse through and gain a deeper understanding of related studies on a particular topic. Additionally, you can use SciSpace Copilot , your AI research assistant, for reading any lengthy research paper and getting a more summarized context of it. A hypothesis can be formed after evaluating many such summarized research papers. Copilot also offers explanations for theories and equations, explains paper in simplified version, allows you to highlight any text in the paper or clip math equations and tables and provides a deeper, clear understanding of what is being said. This can improve the hypothesis by helping you identify potential research gaps.

3. Create a 3-dimensional hypothesis

Variables are an essential part of any reasonable hypothesis. So, identify your independent and dependent variable(s) and form a correlation between them. The ideal way to do this is to write the hypothetical assumption in the ‘if-then' form. If you use this form, make sure that you state the predefined relationship between the variables.

In another way, you can choose to present your hypothesis as a comparison between two variables. Here, you must specify the difference you expect to observe in the results.

4. Write the first draft

Now that everything is in place, it's time to write your hypothesis. For starters, create the first draft. In this version, write what you expect to find from your research.

Clearly separate your independent and dependent variables and the link between them. Don't fixate on syntax at this stage. The goal is to ensure your hypothesis addresses the issue.

5. Proof your hypothesis

After preparing the first draft of your hypothesis, you need to inspect it thoroughly. It should tick all the boxes, like being concise, straightforward, relevant, and accurate. Your final hypothesis has to be well-structured as well.

Research projects are an exciting and crucial part of being a scholar. And once you have your research question, you need a great hypothesis to begin conducting research. Thus, knowing how to write a hypothesis is very important.

Now that you have a firmer grasp on what a good hypothesis constitutes, the different kinds there are, and what process to follow, you will find it much easier to write your hypothesis, which ultimately helps your research.

Now it's easier than ever to streamline your research workflow with SciSpace Discover . Its integrated, comprehensive end-to-end platform for research allows scholars to easily discover, write and publish their research and fosters collaboration.

It includes everything you need, including a repository of over 270 million research papers across disciplines, SEO-optimized summaries and public profiles to show your expertise and experience.

If you found these tips on writing a research hypothesis useful, head over to our blog on Statistical Hypothesis Testing to learn about the top researchers, papers, and institutions in this domain.

Frequently Asked Questions (FAQs)

1. what is the definition of hypothesis.

According to the Oxford dictionary, a hypothesis is defined as “An idea or explanation of something that is based on a few known facts, but that has not yet been proved to be true or correct”.

2. What is an example of hypothesis?

The hypothesis is a statement that proposes a relationship between two or more variables. An example: "If we increase the number of new users who join our platform by 25%, then we will see an increase in revenue."

3. What is an example of null hypothesis?

A null hypothesis is a statement that there is no relationship between two variables. The null hypothesis is written as H0. The null hypothesis states that there is no effect. For example, if you're studying whether or not a particular type of exercise increases strength, your null hypothesis will be "there is no difference in strength between people who exercise and people who don't."

4. What are the types of research?

• Fundamental research

• Applied research

• Qualitative research

• Quantitative research

• Mixed research

• Exploratory research

• Longitudinal research

• Cross-sectional research

• Field research

• Laboratory research

• Fixed research

• Flexible research

• Action research

• Policy research

• Classification research

• Comparative research

• Causal research

• Inductive research

• Deductive research

5. How to write a hypothesis?

• Your hypothesis should be able to predict the relationship and outcome.

• Avoid wordiness by keeping it simple and brief.

• Your hypothesis should contain observable and testable outcomes.

• Your hypothesis should be relevant to the research question.

6. What are the 2 types of hypothesis?

• Null hypotheses are used to test the claim that "there is no difference between two groups of data".

• Alternative hypotheses test the claim that "there is a difference between two data groups".

7. Difference between research question and research hypothesis?

A research question is a broad, open-ended question you will try to answer through your research. A hypothesis is a statement based on prior research or theory that you expect to be true due to your study. Example - Research question: What are the factors that influence the adoption of the new technology? Research hypothesis: There is a positive relationship between age, education and income level with the adoption of the new technology.

8. What is plural for hypothesis?

The plural of hypothesis is hypotheses. Here's an example of how it would be used in a statement, "Numerous well-considered hypotheses are presented in this part, and they are supported by tables and figures that are well-illustrated."

9. What is the red queen hypothesis?

The red queen hypothesis in evolutionary biology states that species must constantly evolve to avoid extinction because if they don't, they will be outcompeted by other species that are evolving. Leigh Van Valen first proposed it in 1973; since then, it has been tested and substantiated many times.

10. Who is known as the father of null hypothesis?

The father of the null hypothesis is Sir Ronald Fisher. He published a paper in 1925 that introduced the concept of null hypothesis testing, and he was also the first to use the term itself.

11. When to reject null hypothesis?

You need to find a significant difference between your two populations to reject the null hypothesis. You can determine that by running statistical tests such as an independent sample t-test or a dependent sample t-test. You should reject the null hypothesis if the p-value is less than 0.05.

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What is a Research Hypothesis: How to Write it, Types, and Examples

Any research begins with a research question and a research hypothesis . A research question alone may not suffice to design the experiment(s) needed to answer it. A hypothesis is central to the scientific method. But what is a hypothesis ? A hypothesis is a testable statement that proposes a possible explanation to a phenomenon, and it may include a prediction. Next, you may ask what is a research hypothesis ? Simply put, a research hypothesis is a prediction or educated guess about the relationship between the variables that you want to investigate.  

It is important to be thorough when developing your research hypothesis. Shortcomings in the framing of a hypothesis can affect the study design and the results. A better understanding of the research hypothesis definition and characteristics of a good hypothesis will make it easier for you to develop your own hypothesis for your research. Let’s dive in to know more about the types of research hypothesis , how to write a research hypothesis , and some research hypothesis examples .  

Table of Contents

What is a hypothesis ?  

A hypothesis is based on the existing body of knowledge in a study area. Framed before the data are collected, a hypothesis states the tentative relationship between independent and dependent variables, along with a prediction of the outcome.  

What is a research hypothesis ?  

Young researchers starting out their journey are usually brimming with questions like “ What is a hypothesis ?” “ What is a research hypothesis ?” “How can I write a good research hypothesis ?”   

A research hypothesis is a statement that proposes a possible explanation for an observable phenomenon or pattern. It guides the direction of a study and predicts the outcome of the investigation. A research hypothesis is testable, i.e., it can be supported or disproven through experimentation or observation.     

Characteristics of a good hypothesis  

Here are the characteristics of a good hypothesis :  

• Clearly formulated and free of language errors and ambiguity  
• Concise and not unnecessarily verbose  
• Has clearly defined variables  
• Testable and stated in a way that allows for it to be disproven  
• Can be tested using a research design that is feasible, ethical, and practical   
• Specific and relevant to the research problem  
• Rooted in a thorough literature search  
• Can generate new knowledge or understanding.  

How to create an effective research hypothesis  

A study begins with the formulation of a research question. A researcher then performs background research. This background information forms the basis for building a good research hypothesis . The researcher then performs experiments, collects, and analyzes the data, interprets the findings, and ultimately, determines if the findings support or negate the original hypothesis.  

Let’s look at each step for creating an effective, testable, and good research hypothesis :  

• Identify a research problem or question: Start by identifying a specific research problem.   
• Review the literature: Conduct an in-depth review of the existing literature related to the research problem to grasp the current knowledge and gaps in the field.   
• Formulate a clear and testable hypothesis : Based on the research question, use existing knowledge to form a clear and testable hypothesis . The hypothesis should state a predicted relationship between two or more variables that can be measured and manipulated. Improve the original draft till it is clear and meaningful.  
• State the null hypothesis: The null hypothesis is a statement that there is no relationship between the variables you are studying.   
• Define the population and sample: Clearly define the population you are studying and the sample you will be using for your research.  
• Select appropriate methods for testing the hypothesis: Select appropriate research methods, such as experiments, surveys, or observational studies, which will allow you to test your research hypothesis .  

Remember that creating a research hypothesis is an iterative process, i.e., you might have to revise it based on the data you collect. You may need to test and reject several hypotheses before answering the research problem.  

How to write a research hypothesis  

When you start writing a research hypothesis , you use an “if–then” statement format, which states the predicted relationship between two or more variables. Clearly identify the independent variables (the variables being changed) and the dependent variables (the variables being measured), as well as the population you are studying. Review and revise your hypothesis as needed.  

An example of a research hypothesis in this format is as follows:  

“ If [athletes] follow [cold water showers daily], then their [endurance] increases.”  

Population: athletes  

Independent variable: daily cold water showers  

Dependent variable: endurance  

You may have understood the characteristics of a good hypothesis . But note that a research hypothesis is not always confirmed; a researcher should be prepared to accept or reject the hypothesis based on the study findings.  

Research hypothesis checklist  

Following from above, here is a 10-point checklist for a good research hypothesis :  

• Testable: A research hypothesis should be able to be tested via experimentation or observation.  
• Specific: A research hypothesis should clearly state the relationship between the variables being studied.  
• Based on prior research: A research hypothesis should be based on existing knowledge and previous research in the field.  
• Falsifiable: A research hypothesis should be able to be disproven through testing.  
• Clear and concise: A research hypothesis should be stated in a clear and concise manner.  
• Logical: A research hypothesis should be logical and consistent with current understanding of the subject.  
• Relevant: A research hypothesis should be relevant to the research question and objectives.  
• Feasible: A research hypothesis should be feasible to test within the scope of the study.  
• Reflects the population: A research hypothesis should consider the population or sample being studied.  
• Uncomplicated: A good research hypothesis is written in a way that is easy for the target audience to understand.  

By following this research hypothesis checklist , you will be able to create a research hypothesis that is strong, well-constructed, and more likely to yield meaningful results.  

Types of research hypothesis  

Different types of research hypothesis are used in scientific research:  

1. Null hypothesis:

A null hypothesis states that there is no change in the dependent variable due to changes to the independent variable. This means that the results are due to chance and are not significant. A null hypothesis is denoted as H0 and is stated as the opposite of what the alternative hypothesis states.   

Example: “ The newly identified virus is not zoonotic .”  

2. Alternative hypothesis:

This states that there is a significant difference or relationship between the variables being studied. It is denoted as H1 or Ha and is usually accepted or rejected in favor of the null hypothesis.  

Example: “ The newly identified virus is zoonotic .”  

3. Directional hypothesis :

This specifies the direction of the relationship or difference between variables; therefore, it tends to use terms like increase, decrease, positive, negative, more, or less.   

Example: “ The inclusion of intervention X decreases infant mortality compared to the original treatment .”   

4. Non-directional hypothesis:

While it does not predict the exact direction or nature of the relationship between the two variables, a non-directional hypothesis states the existence of a relationship or difference between variables but not the direction, nature, or magnitude of the relationship. A non-directional hypothesis may be used when there is no underlying theory or when findings contradict previous research.  

Example, “ Cats and dogs differ in the amount of affection they express .”  

5. Simple hypothesis :

A simple hypothesis only predicts the relationship between one independent and another independent variable.  

Example: “ Applying sunscreen every day slows skin aging .”  

6 . Complex hypothesis :

A complex hypothesis states the relationship or difference between two or more independent and dependent variables.   

Example: “ Applying sunscreen every day slows skin aging, reduces sun burn, and reduces the chances of skin cancer .” (Here, the three dependent variables are slowing skin aging, reducing sun burn, and reducing the chances of skin cancer.)  

7. Associative hypothesis:  

An associative hypothesis states that a change in one variable results in the change of the other variable. The associative hypothesis defines interdependency between variables.  

Example: “ There is a positive association between physical activity levels and overall health .”  

8 . Causal hypothesis:

A causal hypothesis proposes a cause-and-effect interaction between variables.  

Example: “ Long-term alcohol use causes liver damage .”  

Note that some of the types of research hypothesis mentioned above might overlap. The types of hypothesis chosen will depend on the research question and the objective of the study.  

Research hypothesis examples  

Here are some good research hypothesis examples :  

“The use of a specific type of therapy will lead to a reduction in symptoms of depression in individuals with a history of major depressive disorder.”  

“Providing educational interventions on healthy eating habits will result in weight loss in overweight individuals.”  

“Plants that are exposed to certain types of music will grow taller than those that are not exposed to music.”  

“The use of the plant growth regulator X will lead to an increase in the number of flowers produced by plants.”  

Characteristics that make a research hypothesis weak are unclear variables, unoriginality, being too general or too vague, and being untestable. A weak hypothesis leads to weak research and improper methods.   

Some bad research hypothesis examples (and the reasons why they are “bad”) are as follows:  

“This study will show that treatment X is better than any other treatment . ” (This statement is not testable, too broad, and does not consider other treatments that may be effective.)  

“This study will prove that this type of therapy is effective for all mental disorders . ” (This statement is too broad and not testable as mental disorders are complex and different disorders may respond differently to different types of therapy.)  

“Plants can communicate with each other through telepathy . ” (This statement is not testable and lacks a scientific basis.)  

Importance of testable hypothesis  

If a research hypothesis is not testable, the results will not prove or disprove anything meaningful. The conclusions will be vague at best. A testable hypothesis helps a researcher focus on the study outcome and understand the implication of the question and the different variables involved. A testable hypothesis helps a researcher make precise predictions based on prior research.  

To be considered testable, there must be a way to prove that the hypothesis is true or false; further, the results of the hypothesis must be reproducible.  

Frequently Asked Questions (FAQs) on research hypothesis  

1. What is the difference between research question and research hypothesis ?  

A research question defines the problem and helps outline the study objective(s). It is an open-ended statement that is exploratory or probing in nature. Therefore, it does not make predictions or assumptions. It helps a researcher identify what information to collect. A research hypothesis , however, is a specific, testable prediction about the relationship between variables. Accordingly, it guides the study design and data analysis approach.

2. When to reject null hypothesis ?

A null hypothesis should be rejected when the evidence from a statistical test shows that it is unlikely to be true. This happens when the test statistic (e.g., p -value) is less than the defined significance level (e.g., 0.05). Rejecting the null hypothesis does not necessarily mean that the alternative hypothesis is true; it simply means that the evidence found is not compatible with the null hypothesis.  

3. How can I be sure my hypothesis is testable?  

A testable hypothesis should be specific and measurable, and it should state a clear relationship between variables that can be tested with data. To ensure that your hypothesis is testable, consider the following:  

• Clearly define the key variables in your hypothesis. You should be able to measure and manipulate these variables in a way that allows you to test the hypothesis.  
• The hypothesis should predict a specific outcome or relationship between variables that can be measured or quantified.   
• You should be able to collect the necessary data within the constraints of your study.  
• It should be possible for other researchers to replicate your study, using the same methods and variables.   
• Your hypothesis should be testable by using appropriate statistical analysis techniques, so you can draw conclusions, and make inferences about the population from the sample data.  
• The hypothesis should be able to be disproven or rejected through the collection of data.  

4. How do I revise my research hypothesis if my data does not support it?  

If your data does not support your research hypothesis , you will need to revise it or develop a new one. You should examine your data carefully and identify any patterns or anomalies, re-examine your research question, and/or revisit your theory to look for any alternative explanations for your results. Based on your review of the data, literature, and theories, modify your research hypothesis to better align it with the results you obtained. Use your revised hypothesis to guide your research design and data collection. It is important to remain objective throughout the process.  

5. I am performing exploratory research. Do I need to formulate a research hypothesis?  

As opposed to “confirmatory” research, where a researcher has some idea about the relationship between the variables under investigation, exploratory research (or hypothesis-generating research) looks into a completely new topic about which limited information is available. Therefore, the researcher will not have any prior hypotheses. In such cases, a researcher will need to develop a post-hoc hypothesis. A post-hoc research hypothesis is generated after these results are known.  

6. How is a research hypothesis different from a research question?

A research question is an inquiry about a specific topic or phenomenon, typically expressed as a question. It seeks to explore and understand a particular aspect of the research subject. In contrast, a research hypothesis is a specific statement or prediction that suggests an expected relationship between variables. It is formulated based on existing knowledge or theories and guides the research design and data analysis.

7. Can a research hypothesis change during the research process?

Yes, research hypotheses can change during the research process. As researchers collect and analyze data, new insights and information may emerge that require modification or refinement of the initial hypotheses. This can be due to unexpected findings, limitations in the original hypotheses, or the need to explore additional dimensions of the research topic. Flexibility is crucial in research, allowing for adaptation and adjustment of hypotheses to align with the evolving understanding of the subject matter.

8. How many hypotheses should be included in a research study?

The number of research hypotheses in a research study varies depending on the nature and scope of the research. It is not necessary to have multiple hypotheses in every study. Some studies may have only one primary hypothesis, while others may have several related hypotheses. The number of hypotheses should be determined based on the research objectives, research questions, and the complexity of the research topic. It is important to ensure that the hypotheses are focused, testable, and directly related to the research aims.

9. Can research hypotheses be used in qualitative research?

Yes, research hypotheses can be used in qualitative research, although they are more commonly associated with quantitative research. In qualitative research, hypotheses may be formulated as tentative or exploratory statements that guide the investigation. Instead of testing hypotheses through statistical analysis, qualitative researchers may use the hypotheses to guide data collection and analysis, seeking to uncover patterns, themes, or relationships within the qualitative data. The emphasis in qualitative research is often on generating insights and understanding rather than confirming or rejecting specific research hypotheses through statistical testing.

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Hypothesis Testing

When you conduct a piece of quantitative research, you are inevitably attempting to answer a research question or hypothesis that you have set. One method of evaluating this research question is via a process called hypothesis testing , which is sometimes also referred to as significance testing . Since there are many facets to hypothesis testing, we start with the example we refer to throughout this guide.

An example of a lecturer's dilemma

Two statistics lecturers, Sarah and Mike, think that they use the best method to teach their students. Each lecturer has 50 statistics students who are studying a graduate degree in management. In Sarah's class, students have to attend one lecture and one seminar class every week, whilst in Mike's class students only have to attend one lecture. Sarah thinks that seminars, in addition to lectures, are an important teaching method in statistics, whilst Mike believes that lectures are sufficient by themselves and thinks that students are better off solving problems by themselves in their own time. This is the first year that Sarah has given seminars, but since they take up a lot of her time, she wants to make sure that she is not wasting her time and that seminars improve her students' performance.

The research hypothesis

The first step in hypothesis testing is to set a research hypothesis. In Sarah and Mike's study, the aim is to examine the effect that two different teaching methods – providing both lectures and seminar classes (Sarah), and providing lectures by themselves (Mike) – had on the performance of Sarah's 50 students and Mike's 50 students. More specifically, they want to determine whether performance is different between the two different teaching methods. Whilst Mike is skeptical about the effectiveness of seminars, Sarah clearly believes that giving seminars in addition to lectures helps her students do better than those in Mike's class. This leads to the following research hypothesis:

Before moving onto the second step of the hypothesis testing process, we need to take you on a brief detour to explain why you need to run hypothesis testing at all. This is explained next.

Sample to population

If you have measured individuals (or any other type of "object") in a study and want to understand differences (or any other type of effect), you can simply summarize the data you have collected. For example, if Sarah and Mike wanted to know which teaching method was the best, they could simply compare the performance achieved by the two groups of students – the group of students that took lectures and seminar classes, and the group of students that took lectures by themselves – and conclude that the best method was the teaching method which resulted in the highest performance. However, this is generally of only limited appeal because the conclusions could only apply to students in this study. However, if those students were representative of all statistics students on a graduate management degree, the study would have wider appeal.

In statistics terminology, the students in the study are the sample and the larger group they represent (i.e., all statistics students on a graduate management degree) is called the population . Given that the sample of statistics students in the study are representative of a larger population of statistics students, you can use hypothesis testing to understand whether any differences or effects discovered in the study exist in the population. In layman's terms, hypothesis testing is used to establish whether a research hypothesis extends beyond those individuals examined in a single study.

Another example could be taking a sample of 200 breast cancer sufferers in order to test a new drug that is designed to eradicate this type of cancer. As much as you are interested in helping these specific 200 cancer sufferers, your real goal is to establish that the drug works in the population (i.e., all breast cancer sufferers).

As such, by taking a hypothesis testing approach, Sarah and Mike want to generalize their results to a population rather than just the students in their sample. However, in order to use hypothesis testing, you need to re-state your research hypothesis as a null and alternative hypothesis. Before you can do this, it is best to consider the process/structure involved in hypothesis testing and what you are measuring. This structure is presented on the next page .

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How to Write a Research Hypothesis: Good & Bad Examples

What is a research hypothesis?

A research hypothesis is an attempt at explaining a phenomenon or the relationships between phenomena/variables in the real world. Hypotheses are sometimes called “educated guesses”, but they are in fact (or let’s say they should be) based on previous observations, existing theories, scientific evidence, and logic. A research hypothesis is also not a prediction—rather, predictions are ( should be) based on clearly formulated hypotheses. For example, “We tested the hypothesis that KLF2 knockout mice would show deficiencies in heart development” is an assumption or prediction, not a hypothesis. 

The research hypothesis at the basis of this prediction is “the product of the KLF2 gene is involved in the development of the cardiovascular system in mice”—and this hypothesis is probably (hopefully) based on a clear observation, such as that mice with low levels of Kruppel-like factor 2 (which KLF2 codes for) seem to have heart problems. From this hypothesis, you can derive the idea that a mouse in which this particular gene does not function cannot develop a normal cardiovascular system, and then make the prediction that we started with. 

What is the difference between a hypothesis and a prediction?

You might think that these are very subtle differences, and you will certainly come across many publications that do not contain an actual hypothesis or do not make these distinctions correctly. But considering that the formulation and testing of hypotheses is an integral part of the scientific method, it is good to be aware of the concepts underlying this approach. The two hallmarks of a scientific hypothesis are falsifiability (an evaluation standard that was introduced by the philosopher of science Karl Popper in 1934) and testability —if you cannot use experiments or data to decide whether an idea is true or false, then it is not a hypothesis (or at least a very bad one).

So, in a nutshell, you (1) look at existing evidence/theories, (2) come up with a hypothesis, (3) make a prediction that allows you to (4) design an experiment or data analysis to test it, and (5) come to a conclusion. Of course, not all studies have hypotheses (there is also exploratory or hypothesis-generating research), and you do not necessarily have to state your hypothesis as such in your paper. 

But for the sake of understanding the principles of the scientific method, let’s first take a closer look at the different types of hypotheses that research articles refer to and then give you a step-by-step guide for how to formulate a strong hypothesis for your own paper.

Types of Research Hypotheses

Hypotheses can be simple , which means they describe the relationship between one single independent variable (the one you observe variations in or plan to manipulate) and one single dependent variable (the one you expect to be affected by the variations/manipulation). If there are more variables on either side, you are dealing with a complex hypothesis. You can also distinguish hypotheses according to the kind of relationship between the variables you are interested in (e.g., causal or associative ). But apart from these variations, we are usually interested in what is called the “alternative hypothesis” and, in contrast to that, the “null hypothesis”. If you think these two should be listed the other way round, then you are right, logically speaking—the alternative should surely come second. However, since this is the hypothesis we (as researchers) are usually interested in, let’s start from there.

Alternative Hypothesis

If you predict a relationship between two variables in your study, then the research hypothesis that you formulate to describe that relationship is your alternative hypothesis (usually H1 in statistical terms). The goal of your hypothesis testing is thus to demonstrate that there is sufficient evidence that supports the alternative hypothesis, rather than evidence for the possibility that there is no such relationship. The alternative hypothesis is usually the research hypothesis of a study and is based on the literature, previous observations, and widely known theories. 

Null Hypothesis

The hypothesis that describes the other possible outcome, that is, that your variables are not related, is the null hypothesis ( H0 ). Based on your findings, you choose between the two hypotheses—usually that means that if your prediction was correct, you reject the null hypothesis and accept the alternative. Make sure, however, that you are not getting lost at this step of the thinking process: If your prediction is that there will be no difference or change, then you are trying to find support for the null hypothesis and reject H1. 

Directional Hypothesis

While the null hypothesis is obviously “static”, the alternative hypothesis can specify a direction for the observed relationship between variables—for example, that mice with higher expression levels of a certain protein are more active than those with lower levels. This is then called a one-tailed hypothesis. 

Another example for a directional one-tailed alternative hypothesis would be that 

H1: Attending private classes before important exams has a positive effect on performance. 

Your null hypothesis would then be that

H0: Attending private classes before important exams has no/a negative effect on performance.

Nondirectional Hypothesis

A nondirectional hypothesis does not specify the direction of the potentially observed effect, only that there is a relationship between the studied variables—this is called a two-tailed hypothesis. For instance, if you are studying a new drug that has shown some effects on pathways involved in a certain condition (e.g., anxiety) in vitro in the lab, but you can’t say for sure whether it will have the same effects in an animal model or maybe induce other/side effects that you can’t predict and potentially increase anxiety levels instead, you could state the two hypotheses like this:

H1: The only lab-tested drug (somehow) affects anxiety levels in an anxiety mouse model.

You then test this nondirectional alternative hypothesis against the null hypothesis:

H0: The only lab-tested drug has no effect on anxiety levels in an anxiety mouse model.

How to Write a Hypothesis for a Research Paper

Now that we understand the important distinctions between different kinds of research hypotheses, let’s look at a simple process of how to write a hypothesis.

Writing a Hypothesis Step:1

Ask a question, based on earlier research. Research always starts with a question, but one that takes into account what is already known about a topic or phenomenon. For example, if you are interested in whether people who have pets are happier than those who don’t, do a literature search and find out what has already been demonstrated. You will probably realize that yes, there is quite a bit of research that shows a relationship between happiness and owning a pet—and even studies that show that owning a dog is more beneficial than owning a cat ! Let’s say you are so intrigued by this finding that you wonder: 

What is it that makes dog owners even happier than cat owners? 

Let’s move on to Step 2 and find an answer to that question.

Writing a Hypothesis Step 2:

Formulate a strong hypothesis by answering your own question. Again, you don’t want to make things up, take unicorns into account, or repeat/ignore what has already been done. Looking at the dog-vs-cat papers your literature search returned, you see that most studies are based on self-report questionnaires on personality traits, mental health, and life satisfaction. What you don’t find is any data on actual (mental or physical) health measures, and no experiments. You therefore decide to make a bold claim come up with the carefully thought-through hypothesis that it’s maybe the lifestyle of the dog owners, which includes walking their dog several times per day, engaging in fun and healthy activities such as agility competitions, and taking them on trips, that gives them that extra boost in happiness. You could therefore answer your question in the following way:

Dog owners are happier than cat owners because of the dog-related activities they engage in.

Now you have to verify that your hypothesis fulfills the two requirements we introduced at the beginning of this resource article: falsifiability and testability . If it can’t be wrong and can’t be tested, it’s not a hypothesis. We are lucky, however, because yes, we can test whether owning a dog but not engaging in any of those activities leads to lower levels of happiness or well-being than owning a dog and playing and running around with them or taking them on trips.  

Writing a Hypothesis Step 3:

Make your predictions and define your variables. We have verified that we can test our hypothesis, but now we have to define all the relevant variables, design our experiment or data analysis, and make precise predictions. You could, for example, decide to study dog owners (not surprising at this point), let them fill in questionnaires about their lifestyle as well as their life satisfaction (as other studies did), and then compare two groups of active and inactive dog owners. Alternatively, if you want to go beyond the data that earlier studies produced and analyzed and directly manipulate the activity level of your dog owners to study the effect of that manipulation, you could invite them to your lab, select groups of participants with similar lifestyles, make them change their lifestyle (e.g., couch potato dog owners start agility classes, very active ones have to refrain from any fun activities for a certain period of time) and assess their happiness levels before and after the intervention. In both cases, your independent variable would be “ level of engagement in fun activities with dog” and your dependent variable would be happiness or well-being . 

Examples of a Good and Bad Hypothesis

Let’s look at a few examples of good and bad hypotheses to get you started.

Good Hypothesis Examples

Bad Hypothesis Examples

Tips for Writing a Research Hypothesis

If you understood the distinction between a hypothesis and a prediction we made at the beginning of this article, then you will have no problem formulating your hypotheses and predictions correctly. To refresh your memory: We have to (1) look at existing evidence, (2) come up with a hypothesis, (3) make a prediction, and (4) design an experiment. For example, you could summarize your dog/happiness study like this:

(1) While research suggests that dog owners are happier than cat owners, there are no reports on what factors drive this difference. (2) We hypothesized that it is the fun activities that many dog owners (but very few cat owners) engage in with their pets that increases their happiness levels. (3) We thus predicted that preventing very active dog owners from engaging in such activities for some time and making very inactive dog owners take up such activities would lead to an increase and decrease in their overall self-ratings of happiness, respectively. (4) To test this, we invited dog owners into our lab, assessed their mental and emotional well-being through questionnaires, and then assigned them to an “active” and an “inactive” group, depending on… 

Note that you use “we hypothesize” only for your hypothesis, not for your experimental prediction, and “would” or “if – then” only for your prediction, not your hypothesis. A hypothesis that states that something “would” affect something else sounds as if you don’t have enough confidence to make a clear statement—in which case you can’t expect your readers to believe in your research either. Write in the present tense, don’t use modal verbs that express varying degrees of certainty (such as may, might, or could ), and remember that you are not drawing a conclusion while trying not to exaggerate but making a clear statement that you then, in a way, try to disprove . And if that happens, that is not something to fear but an important part of the scientific process.

Similarly, don’t use “we hypothesize” when you explain the implications of your research or make predictions in the conclusion section of your manuscript, since these are clearly not hypotheses in the true sense of the word. As we said earlier, you will find that many authors of academic articles do not seem to care too much about these rather subtle distinctions, but thinking very clearly about your own research will not only help you write better but also ensure that even that infamous Reviewer 2 will find fewer reasons to nitpick about your manuscript. 

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On the Wordvice academic resources website , you can also find many more articles and other resources that can help you with writing the other parts of your research paper , with making a research paper outline before you put everything together, or with writing an effective cover letter once you are ready to submit.

What Is A Research (Scientific) Hypothesis? A plain-language explainer + examples

By:  Derek Jansen (MBA)  | Reviewed By: Dr Eunice Rautenbach | June 2020

If you’re new to the world of research, or it’s your first time writing a dissertation or thesis, you’re probably noticing that the words “research hypothesis” and “scientific hypothesis” are used quite a bit, and you’re wondering what they mean in a research context .

“Hypothesis” is one of those words that people use loosely, thinking they understand what it means. However, it has a very specific meaning within academic research. So, it’s important to understand the exact meaning before you start hypothesizing. 

Research Hypothesis 101

• What is a hypothesis ?
• What is a research hypothesis (scientific hypothesis)?
• Requirements for a research hypothesis
• Definition of a research hypothesis
• The null hypothesis

What is a hypothesis?

Let’s start with the general definition of a hypothesis (not a research hypothesis or scientific hypothesis), according to the Cambridge Dictionary:

Hypothesis: an idea or explanation for something that is based on known facts but has not yet been proved.

In other words, it’s a statement that provides an explanation for why or how something works, based on facts (or some reasonable assumptions), but that has not yet been specifically tested . For example, a hypothesis might look something like this:

Hypothesis: sleep impacts academic performance.

This statement predicts that academic performance will be influenced by the amount and/or quality of sleep a student engages in – sounds reasonable, right? It’s based on reasonable assumptions , underpinned by what we currently know about sleep and health (from the existing literature). So, loosely speaking, we could call it a hypothesis, at least by the dictionary definition.

But that’s not good enough…

Unfortunately, that’s not quite sophisticated enough to describe a research hypothesis (also sometimes called a scientific hypothesis), and it wouldn’t be acceptable in a dissertation, thesis or research paper . In the world of academic research, a statement needs a few more criteria to constitute a true research hypothesis .

What is a research hypothesis?

A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes – specificity , clarity and testability .

Let’s take a look at these more closely.

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Hypothesis Essential #1: Specificity & Clarity

A good research hypothesis needs to be extremely clear and articulate about both what’ s being assessed (who or what variables are involved ) and the expected outcome (for example, a difference between groups, a relationship between variables, etc.).

Let’s stick with our sleepy students example and look at how this statement could be more specific and clear.

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

As you can see, the statement is very specific as it identifies the variables involved (sleep hours and test grades), the parties involved (two groups of students), as well as the predicted relationship type (a positive relationship). There’s no ambiguity or uncertainty about who or what is involved in the statement, and the expected outcome is clear.

Contrast that to the original hypothesis we looked at – “Sleep impacts academic performance” – and you can see the difference. “Sleep” and “academic performance” are both comparatively vague , and there’s no indication of what the expected relationship direction is (more sleep or less sleep). As you can see, specificity and clarity are key.

Hypothesis Essential #2: Testability (Provability)

A statement must be testable to qualify as a research hypothesis. In other words, there needs to be a way to prove (or disprove) the statement. If it’s not testable, it’s not a hypothesis – simple as that.

For example, consider the hypothesis we mentioned earlier:

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.  

We could test this statement by undertaking a quantitative study involving two groups of students, one that gets 8 or more hours of sleep per night for a fixed period, and one that gets less. We could then compare the standardised test results for both groups to see if there’s a statistically significant difference. 

Again, if you compare this to the original hypothesis we looked at – “Sleep impacts academic performance” – you can see that it would be quite difficult to test that statement, primarily because it isn’t specific enough. How much sleep? By who? What type of academic performance?

So, remember the mantra – if you can’t test it, it’s not a hypothesis 🙂

Defining A Research Hypothesis

You’re still with us? Great! Let’s recap and pin down a clear definition of a hypothesis.

A research hypothesis (or scientific hypothesis) is a statement about an expected relationship between variables, or explanation of an occurrence, that is clear, specific and testable.

So, when you write up hypotheses for your dissertation or thesis, make sure that they meet all these criteria. If you do, you’ll not only have rock-solid hypotheses but you’ll also ensure a clear focus for your entire research project.

What about the null hypothesis?

You may have also heard the terms null hypothesis , alternative hypothesis, or H-zero thrown around. At a simple level, the null hypothesis is the counter-proposal to the original hypothesis.

For example, if the hypothesis predicts that there is a relationship between two variables (for example, sleep and academic performance), the null hypothesis would predict that there is no relationship between those variables.

At a more technical level, the null hypothesis proposes that no statistical significance exists in a set of given observations and that any differences are due to chance alone.

And there you have it – hypotheses in a nutshell. 

If you have any questions, be sure to leave a comment below and we’ll do our best to help you. If you need hands-on help developing and testing your hypotheses, consider our private coaching service , where we hold your hand through the research journey.

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17 Comments

Very useful information. I benefit more from getting more information in this regard.

Very great insight,educative and informative. Please give meet deep critics on many research data of public international Law like human rights, environment, natural resources, law of the sea etc

In a book I read a distinction is made between null, research, and alternative hypothesis. As far as I understand, alternative and research hypotheses are the same. Can you please elaborate? Best Afshin

This is a self explanatory, easy going site. I will recommend this to my friends and colleagues.

Very good definition. How can I cite your definition in my thesis? Thank you. Is nul hypothesis compulsory in a research?

It’s a counter-proposal to be proven as a rejection

Please what is the difference between alternate hypothesis and research hypothesis?

It is a very good explanation. However, it limits hypotheses to statistically tasteable ideas. What about for qualitative researches or other researches that involve quantitative data that don’t need statistical tests?

In qualitative research, one typically uses propositions, not hypotheses.

could you please elaborate it more

I’ve benefited greatly from these notes, thank you.

This is very helpful

well articulated ideas are presented here, thank you for being reliable sources of information

Excellent. Thanks for being clear and sound about the research methodology and hypothesis (quantitative research)

I have only a simple question regarding the null hypothesis. – Is the null hypothesis (Ho) known as the reversible hypothesis of the alternative hypothesis (H1? – How to test it in academic research?

this is very important note help me much more

Hi” best wishes to you and your very nice blog” 

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Research Hypothesis: What It Is, Types + How to Develop?

A research study starts with a question. Researchers worldwide ask questions and create research hypotheses. The effectiveness of research relies on developing a good research hypothesis. Examples of research hypotheses can guide researchers in writing effective ones.

In this blog, we’ll learn what a research hypothesis is, why it’s important in research, and the different types used in science. We’ll also guide you through creating your research hypothesis and discussing ways to test and evaluate it.

What is a Research Hypothesis?

A hypothesis is like a guess or idea that you suggest to check if it’s true. A research hypothesis is a statement that brings up a question and predicts what might happen.

It’s really important in the scientific method and is used in experiments to figure things out. Essentially, it’s an educated guess about how things are connected in the research.

A research hypothesis usually includes pointing out the independent variable (the thing they’re changing or studying) and the dependent variable (the result they’re measuring or watching). It helps plan how to gather and analyze data to see if there’s evidence to support or deny the expected connection between these variables.

Importance of Hypothesis in Research

Hypotheses are really important in research. They help design studies, allow for practical testing, and add to our scientific knowledge. Their main role is to organize research projects, making them purposeful, focused, and valuable to the scientific community. Let’s look at some key reasons why they matter:

• A research hypothesis helps test theories.

A hypothesis plays a pivotal role in the scientific method by providing a basis for testing existing theories. For example, a hypothesis might test the predictive power of a psychological theory on human behavior.

• It serves as a great platform for investigation activities.

It serves as a launching pad for investigation activities, which offers researchers a clear starting point. A research hypothesis can explore the relationship between exercise and stress reduction.

• Hypothesis guides the research work or study.

A well-formulated hypothesis guides the entire research process. It ensures that the study remains focused and purposeful. For instance, a hypothesis about the impact of social media on interpersonal relationships provides clear guidance for a study.

• Hypothesis sometimes suggests theories.

In some cases, a hypothesis can suggest new theories or modifications to existing ones. For example, a hypothesis testing the effectiveness of a new drug might prompt a reconsideration of current medical theories.

• It helps in knowing the data needs.

A hypothesis clarifies the data requirements for a study, ensuring that researchers collect the necessary information—a hypothesis guiding the collection of demographic data to analyze the influence of age on a particular phenomenon.

• The hypothesis explains social phenomena.

Hypotheses are instrumental in explaining complex social phenomena. For instance, a hypothesis might explore the relationship between economic factors and crime rates in a given community.

• Hypothesis provides a relationship between phenomena for empirical Testing.

Hypotheses establish clear relationships between phenomena, paving the way for empirical testing. An example could be a hypothesis exploring the correlation between sleep patterns and academic performance.

• It helps in knowing the most suitable analysis technique.

A hypothesis guides researchers in selecting the most appropriate analysis techniques for their data. For example, a hypothesis focusing on the effectiveness of a teaching method may lead to the choice of statistical analyses best suited for educational research.

Characteristics of a Good Research Hypothesis

A hypothesis is a specific idea that you can test in a study. It often comes from looking at past research and theories. A good hypothesis usually starts with a research question that you can explore through background research. For it to be effective, consider these key characteristics:

• Clear and Focused Language: A good hypothesis uses clear and focused language to avoid confusion and ensure everyone understands it.
• Related to the Research Topic: The hypothesis should directly relate to the research topic, acting as a bridge between the specific question and the broader study.
• Testable: An effective hypothesis can be tested, meaning its prediction can be checked with real data to support or challenge the proposed relationship.
• Potential for Exploration: A good hypothesis often comes from a research question that invites further exploration. Doing background research helps find gaps and potential areas to investigate.
• Includes Variables: The hypothesis should clearly state both the independent and dependent variables, specifying the factors being studied and the expected outcomes.
• Ethical Considerations: Check if variables can be manipulated without breaking ethical standards. It’s crucial to maintain ethical research practices.
• Predicts Outcomes: The hypothesis should predict the expected relationship and outcome, acting as a roadmap for the study and guiding data collection and analysis.
• Simple and Concise: A good hypothesis avoids unnecessary complexity and is simple and concise, expressing the essence of the proposed relationship clearly.
• Clear and Assumption-Free: The hypothesis should be clear and free from assumptions about the reader’s prior knowledge, ensuring universal understanding.
• Observable and Testable Results: A strong hypothesis implies research that produces observable and testable results, making sure the study’s outcomes can be effectively measured and analyzed.

When you use these characteristics as a checklist, it can help you create a good research hypothesis. It’ll guide improving and strengthening the hypothesis, identifying any weaknesses, and making necessary changes. Crafting a hypothesis with these features helps you conduct a thorough and insightful research study.

Types of Research Hypotheses

The research hypothesis comes in various types, each serving a specific purpose in guiding the scientific investigation. Knowing the differences will make it easier for you to create your own hypothesis. Here’s an overview of the common types:

01. Null Hypothesis

The null hypothesis states that there is no connection between two considered variables or that two groups are unrelated. As discussed earlier, a hypothesis is an unproven assumption lacking sufficient supporting data. It serves as the statement researchers aim to disprove. It is testable, verifiable, and can be rejected.

For example, if you’re studying the relationship between Project A and Project B, assuming both projects are of equal standard is your null hypothesis. It needs to be specific for your study.

02. Alternative Hypothesis

The alternative hypothesis is basically another option to the null hypothesis. It involves looking for a significant change or alternative that could lead you to reject the null hypothesis. It’s a different idea compared to the null hypothesis.

When you create a null hypothesis, you’re making an educated guess about whether something is true or if there’s a connection between that thing and another variable. If the null view suggests something is correct, the alternative hypothesis says it’s incorrect. 

For instance, if your null hypothesis is “I’m going to be $1000 richer,” the alternative hypothesis would be “I’m not going to get $1000 or be richer.”

03. Directional Hypothesis

The directional hypothesis predicts the direction of the relationship between independent and dependent variables. They specify whether the effect will be positive or negative.

If you increase your study hours, you will experience a positive association with your exam scores. This hypothesis suggests that as you increase the independent variable (study hours), there will also be an increase in the dependent variable (exam scores).

04. Non-directional Hypothesis

The non-directional hypothesis predicts the existence of a relationship between variables but does not specify the direction of the effect. It suggests that there will be a significant difference or relationship, but it does not predict the nature of that difference.

For example, you will find no notable difference in test scores between students who receive the educational intervention and those who do not. However, once you compare the test scores of the two groups, you will notice an important difference.

05. Simple Hypothesis

A simple hypothesis predicts a relationship between one dependent variable and one independent variable without specifying the nature of that relationship. It’s simple and usually used when we don’t know much about how the two things are connected.

For example, if you adopt effective study habits, you will achieve higher exam scores than those with poor study habits.

06. Complex Hypothesis

A complex hypothesis is an idea that specifies a relationship between multiple independent and dependent variables. It is a more detailed idea than a simple hypothesis.

While a simple view suggests a straightforward cause-and-effect relationship between two things, a complex hypothesis involves many factors and how they’re connected to each other.

For example, when you increase your study time, you tend to achieve higher exam scores. The connection between your study time and exam performance is affected by various factors, including the quality of your sleep, your motivation levels, and the effectiveness of your study techniques.

If you sleep well, stay highly motivated, and use effective study strategies, you may observe a more robust positive correlation between the time you spend studying and your exam scores, unlike those who may lack these factors.

07. Associative Hypothesis

An associative hypothesis proposes a connection between two things without saying that one causes the other. Basically, it suggests that when one thing changes, the other changes too, but it doesn’t claim that one thing is causing the change in the other.

For example, you will likely notice higher exam scores when you increase your study time. You can recognize an association between your study time and exam scores in this scenario.

Your hypothesis acknowledges a relationship between the two variables—your study time and exam scores—without asserting that increased study time directly causes higher exam scores. You need to consider that other factors, like motivation or learning style, could affect the observed association.

08. Causal Hypothesis

A causal hypothesis proposes a cause-and-effect relationship between two variables. It suggests that changes in one variable directly cause changes in another variable.

For example, when you increase your study time, you experience higher exam scores. This hypothesis suggests a direct cause-and-effect relationship, indicating that the more time you spend studying, the higher your exam scores. It assumes that changes in your study time directly influence changes in your exam performance.

09. Empirical Hypothesis

An empirical hypothesis is a statement based on things we can see and measure. It comes from direct observation or experiments and can be tested with real-world evidence. If an experiment proves a theory, it supports the idea and shows it’s not just a guess. This makes the statement more reliable than a wild guess.

For example, if you increase the dosage of a certain medication, you might observe a quicker recovery time for patients. Imagine you’re in charge of a clinical trial. In this trial, patients are given varying dosages of the medication, and you measure and compare their recovery times. This allows you to directly see the effects of different dosages on how fast patients recover.

This way, you can create a research hypothesis: “Increasing the dosage of a certain medication will lead to a faster recovery time for patients.”

10. Statistical Hypothesis

A statistical hypothesis is a statement or assumption about a population parameter that is the subject of an investigation. It serves as the basis for statistical analysis and testing. It is often tested using statistical methods to draw inferences about the larger population.

In a hypothesis test, statistical evidence is collected to either reject the null hypothesis in favor of the alternative hypothesis or fail to reject the null hypothesis due to insufficient evidence.

For example, let’s say you’re testing a new medicine. Your hypothesis could be that the medicine doesn’t really help patients get better. So, you collect data and use statistics to see if your guess is right or if the medicine actually makes a difference.

If the data strongly shows that the medicine does help, you say your guess was wrong, and the medicine does make a difference. But if the proof isn’t strong enough, you can stick with your original guess because you didn’t get enough evidence to change your mind.

How to Develop a Research Hypotheses?

Step 1: identify your research problem or topic..

Define the area of interest or the problem you want to investigate. Make sure it’s clear and well-defined.

Start by asking a question about your chosen topic. Consider the limitations of your research and create a straightforward problem related to your topic. Once you’ve done that, you can develop and test a hypothesis with evidence.

Step 2: Conduct a literature review

Review existing literature related to your research problem. This will help you understand the current state of knowledge in the field, identify gaps, and build a foundation for your hypothesis. Consider the following questions:

• What existing research has been conducted on your chosen topic?
• Are there any gaps or unanswered questions in the current literature?
• How will the existing literature contribute to the foundation of your research?

Step 3: Formulate your research question

Based on your literature review, create a specific and concise research question that addresses your identified problem. Your research question should be clear, focused, and relevant to your field of study.

Step 4: Identify variables

Determine the key variables involved in your research question. Variables are the factors or phenomena that you will study and manipulate to test your hypothesis.

• Independent Variable: The variable you manipulate or control.
• Dependent Variable: The variable you measure to observe the effect of the independent variable.

Step 5: State the Null hypothesis

The null hypothesis is a statement that there is no significant difference or effect. It serves as a baseline for comparison with the alternative hypothesis.

Step 6: Select appropriate methods for testing the hypothesis

Choose research methods that align with your study objectives, such as experiments, surveys, or observational studies. The selected methods enable you to test your research hypothesis effectively.

Creating a research hypothesis usually takes more than one try. Expect to make changes as you collect data. It’s normal to test and say no to a few hypotheses before you find the right answer to your research question.

Testing and Evaluating Hypotheses

Testing hypotheses is a really important part of research. It’s like the practical side of things. Here, real-world evidence will help you determine how different things are connected. Let’s explore the main steps in hypothesis testing:

• State your research hypothesis.

Before testing, clearly articulate your research hypothesis. This involves framing both a null hypothesis, suggesting no significant effect or relationship, and an alternative hypothesis, proposing the expected outcome.

• Collect data strategically.

Plan how you will gather information in a way that fits your study. Make sure your data collection method matches the things you’re studying.

Whether through surveys, observations, or experiments, this step demands precision and adherence to the established methodology. The quality of data collected directly influences the credibility of study outcomes.

• Perform an appropriate statistical test.

Choose a statistical test that aligns with the nature of your data and the hypotheses being tested. Whether it’s a t-test, chi-square test, ANOVA, or regression analysis, selecting the right statistical tool is paramount for accurate and reliable results.

• Decide if your idea was right or wrong.

Following the statistical analysis, evaluate the results in the context of your null hypothesis. You need to decide if you should reject your null hypothesis or not.

• Share what you found.

When discussing what you found in your research, be clear and organized. Say whether your idea was supported or not, and talk about what your results mean. Also, mention any limits to your study and suggest ideas for future research.

The Role of QuestionPro to Develop a Good Research Hypothesis

QuestionPro is a survey and research platform that provides tools for creating, distributing, and analyzing surveys. It plays a crucial role in the research process, especially when you’re in the initial stages of hypothesis development. Here’s how QuestionPro can help you to develop a good research hypothesis:

• Survey design and data collection: You can use the platform to create targeted questions that help you gather relevant data.
• Exploratory research: Through surveys and feedback mechanisms on QuestionPro, you can conduct exploratory research to understand the landscape of a particular subject.
• Literature review and background research: QuestionPro surveys can collect sample population opinions, experiences, and preferences. This data and a thorough literature evaluation can help you generate a well-grounded hypothesis by improving your research knowledge.
• Identifying variables: Using targeted survey questions, you can identify relevant variables related to their research topic.
• Testing assumptions: You can use surveys to informally test certain assumptions or hypotheses before formalizing a research hypothesis.
• Data analysis tools: QuestionPro provides tools for analyzing survey data. You can use these tools to identify the collected data’s patterns, correlations, or trends.
• Refining your hypotheses: As you collect data through QuestionPro, you can adjust your hypotheses based on the real-world responses you receive.

A research hypothesis is like a guide for researchers in science. It’s a well-thought-out idea that has been thoroughly tested. This idea is crucial as researchers can explore different fields, such as medicine, social sciences, and natural sciences. The research hypothesis links theories to real-world evidence and gives researchers a clear path to explore and make discoveries.

QuestionPro Research Suite is a helpful tool for researchers. It makes creating surveys, collecting data, and analyzing information easily. It supports all kinds of research, from exploring new ideas to forming hypotheses. With a focus on using data, it helps researchers do their best work.

Are you interested in learning more about QuestionPro Research Suite? Take advantage of QuestionPro’s free trial to get an initial look at its capabilities and realize the full potential of your research efforts.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

How to Write a Hypothesis: A Step-by-Step Guide

Introduction

An overview of the research hypothesis, different types of hypotheses, variables in a hypothesis, how to formulate an effective research hypothesis, designing a study around your hypothesis.

The scientific method can derive and test predictions as hypotheses. Empirical research can then provide support (or lack thereof) for the hypotheses. Even failure to find support for a hypothesis still represents a valuable contribution to scientific knowledge. Let's look more closely at the idea of the hypothesis and the role it plays in research.

As much as the term exists in everyday language, there is a detailed development that informs the word "hypothesis" when applied to research. A good research hypothesis is informed by prior research and guides research design and data analysis , so it is important to understand how a hypothesis is defined and understood by researchers.

What is the simple definition of a hypothesis?

A hypothesis is a testable prediction about an outcome between two or more variables . It functions as a navigational tool in the research process, directing what you aim to predict and how.

What is the hypothesis for in research?

In research, a hypothesis serves as the cornerstone for your empirical study. It not only lays out what you aim to investigate but also provides a structured approach for your data collection and analysis.

Essentially, it bridges the gap between the theoretical and the empirical, guiding your investigation throughout its course.

What is an example of a hypothesis?

If you are studying the relationship between physical exercise and mental health, a suitable hypothesis could be: "Regular physical exercise leads to improved mental well-being among adults."

This statement constitutes a specific and testable hypothesis that directly relates to the variables you are investigating.

What makes a good hypothesis?

A good hypothesis possesses several key characteristics. Firstly, it must be testable, allowing you to analyze data through empirical means, such as observation or experimentation, to assess if there is significant support for the hypothesis. Secondly, a hypothesis should be specific and unambiguous, giving a clear understanding of the expected relationship between variables. Lastly, it should be grounded in existing research or theoretical frameworks , ensuring its relevance and applicability.

Understanding the types of hypotheses can greatly enhance how you construct and work with hypotheses. While all hypotheses serve the essential function of guiding your study, there are varying purposes among the types of hypotheses. In addition, all hypotheses stand in contrast to the null hypothesis, or the assumption that there is no significant relationship between the variables .

Here, we explore various kinds of hypotheses to provide you with the tools needed to craft effective hypotheses for your specific research needs. Bear in mind that many of these hypothesis types may overlap with one another, and the specific type that is typically used will likely depend on the area of research and methodology you are following.

Null hypothesis

The null hypothesis is a statement that there is no effect or relationship between the variables being studied. In statistical terms, it serves as the default assumption that any observed differences are due to random chance.

For example, if you're studying the effect of a drug on blood pressure, the null hypothesis might state that the drug has no effect.

Alternative hypothesis

Contrary to the null hypothesis, the alternative hypothesis suggests that there is a significant relationship or effect between variables.

Using the drug example, the alternative hypothesis would posit that the drug does indeed affect blood pressure. This is what researchers aim to prove.

Simple hypothesis

A simple hypothesis makes a prediction about the relationship between two variables, and only two variables.

For example, "Increased study time results in better exam scores." Here, "study time" and "exam scores" are the only variables involved.

Complex hypothesis

A complex hypothesis, as the name suggests, involves more than two variables. For instance, "Increased study time and access to resources result in better exam scores." Here, "study time," "access to resources," and "exam scores" are all variables.

This hypothesis refers to multiple potential mediating variables. Other hypotheses could also include predictions about variables that moderate the relationship between the independent variable and dependent variable .

Directional hypothesis

A directional hypothesis specifies the direction of the expected relationship between variables. For example, "Eating more fruits and vegetables leads to a decrease in heart disease."

Here, the direction of heart disease is explicitly predicted to decrease, due to effects from eating more fruits and vegetables. All hypotheses typically specify the expected direction of the relationship between the independent and dependent variable, such that researchers can test if this prediction holds in their data analysis .

Statistical hypothesis

A statistical hypothesis is one that is testable through statistical methods, providing a numerical value that can be analyzed. This is commonly seen in quantitative research .

For example, "There is a statistically significant difference in test scores between students who study for one hour and those who study for two."

Empirical hypothesis

An empirical hypothesis is derived from observations and is tested through empirical methods, often through experimentation or survey data . Empirical hypotheses may also be assessed with statistical analyses.

For example, "Regular exercise is correlated with a lower incidence of depression," could be tested through surveys that measure exercise frequency and depression levels.

Causal hypothesis

A causal hypothesis proposes that one variable causes a change in another. This type of hypothesis is often tested through controlled experiments.

For example, "Smoking causes lung cancer," assumes a direct causal relationship.

Associative hypothesis

Unlike causal hypotheses, associative hypotheses suggest a relationship between variables but do not imply causation.

For instance, "People who smoke are more likely to get lung cancer," notes an association but doesn't claim that smoking causes lung cancer directly.

Relational hypothesis

A relational hypothesis explores the relationship between two or more variables but doesn't specify the nature of the relationship.

For example, "There is a relationship between diet and heart health," leaves the nature of the relationship (causal, associative, etc.) open to interpretation.

Logical hypothesis

A logical hypothesis is based on sound reasoning and logical principles. It's often used in theoretical research to explore abstract concepts, rather than being based on empirical data.

For example, "If all men are mortal and Socrates is a man, then Socrates is mortal," employs logical reasoning to make its point.

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In any research hypothesis, variables play a critical role. These are the elements or factors that the researcher manipulates, controls, or measures. Understanding variables is essential for crafting a clear, testable hypothesis and for the stages of research that follow, such as data collection and analysis.

In the realm of hypotheses, there are generally two types of variables to consider: independent and dependent. Independent variables are what you, as the researcher, manipulate or change in your study. It's considered the cause in the relationship you're investigating. For instance, in a study examining the impact of sleep duration on academic performance, the independent variable would be the amount of sleep participants get.

Conversely, the dependent variable is the outcome you measure to gauge the effect of your manipulation. It's the effect in the cause-and-effect relationship. The dependent variable thus refers to the main outcome of interest in your study. In the same sleep study example, the academic performance, perhaps measured by exam scores or GPA, would be the dependent variable.

Beyond these two primary types, you might also encounter control variables. These are variables that could potentially influence the outcome and are therefore kept constant to isolate the relationship between the independent and dependent variables . For example, in the sleep and academic performance study, control variables could include age, diet, or even the subject of study.

By clearly identifying and understanding the roles of these variables in your hypothesis, you set the stage for a methodologically sound research project. It helps you develop focused research questions, design appropriate experiments or observations, and carry out meaningful data analysis . It's a step that lays the groundwork for the success of your entire study.

Crafting a strong, testable hypothesis is crucial for the success of any research project. It sets the stage for everything from your study design to data collection and analysis . Below are some key considerations to keep in mind when formulating your hypothesis:

• Be specific : A vague hypothesis can lead to ambiguous results and interpretations . Clearly define your variables and the expected relationship between them.
• Ensure testability : A good hypothesis should be testable through empirical means, whether by observation , experimentation, or other forms of data analysis.
• Ground in literature : Before creating your hypothesis, consult existing research and theories. This not only helps you identify gaps in current knowledge but also gives you valuable context and credibility for crafting your hypothesis.
• Use simple language : While your hypothesis should be conceptually sound, it doesn't have to be complicated. Aim for clarity and simplicity in your wording.
• State direction, if applicable : If your hypothesis involves a directional outcome (e.g., "increase" or "decrease"), make sure to specify this. You also need to think about how you will measure whether or not the outcome moved in the direction you predicted.
• Keep it focused : One of the common pitfalls in hypothesis formulation is trying to answer too many questions at once. Keep your hypothesis focused on a specific issue or relationship.
• Account for control variables : Identify any variables that could potentially impact the outcome and consider how you will control for them in your study.
• Be ethical : Make sure your hypothesis and the methods for testing it comply with ethical standards , particularly if your research involves human or animal subjects.

Designing your study involves multiple key phases that help ensure the rigor and validity of your research. Here we discuss these crucial components in more detail.

Literature review

Starting with a comprehensive literature review is essential. This step allows you to understand the existing body of knowledge related to your hypothesis and helps you identify gaps that your research could fill. Your research should aim to contribute some novel understanding to existing literature, and your hypotheses can reflect this. A literature review also provides valuable insights into how similar research projects were executed, thereby helping you fine-tune your own approach.

Research methods

Choosing the right research methods is critical. Whether it's a survey, an experiment, or observational study, the methodology should be the most appropriate for testing your hypothesis. Your choice of methods will also depend on whether your research is quantitative, qualitative, or mixed-methods. Make sure the chosen methods align well with the variables you are studying and the type of data you need.

Preliminary research

Before diving into a full-scale study, it’s often beneficial to conduct preliminary research or a pilot study . This allows you to test your research methods on a smaller scale, refine your tools, and identify any potential issues. For instance, a pilot survey can help you determine if your questions are clear and if the survey effectively captures the data you need. This step can save you both time and resources in the long run.

Data analysis

Finally, planning your data analysis in advance is crucial for a successful study. Decide which statistical or analytical tools are most suited for your data type and research questions . For quantitative research, you might opt for t-tests, ANOVA, or regression analyses. For qualitative research , thematic analysis or grounded theory may be more appropriate. This phase is integral for interpreting your results and drawing meaningful conclusions in relation to your research question.

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How to write a research hypothesis

Last updated

19 January 2023

Reviewed by

Miroslav Damyanov

Start with a broad subject matter that excites you, so your curiosity will motivate your work. Conduct a literature search to determine the range of questions already addressed and spot any holes in the existing research.

Narrow the topics that interest you and determine your research question. Rather than focusing on a hole in the research, you might choose to challenge an existing assumption, a process called problematization. You may also find yourself with a short list of questions or related topics.

Use the FINER method to determine the single problem you'll address with your research. FINER stands for:

I nteresting

You need a feasible research question, meaning that there is a way to address the question. You should find it interesting, but so should a larger audience. Rather than repeating research that others have already conducted, your research hypothesis should test something novel or unique. 

The research must fall into accepted ethical parameters as defined by the government of your country and your university or college if you're an academic. You'll also need to come up with a relevant question since your research should provide a contribution to the existing research area.

This process typically narrows your shortlist down to a single problem you'd like to study and the variable you want to test. You're ready to write your hypothesis statements.

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• Types of research hypotheses

It is important to narrow your topic down to one idea before trying to write your research hypothesis. You'll only test one problem at a time. To do this, you'll write two hypotheses – a null hypothesis (H0) and an alternative hypothesis (Ha).

You'll come across many terms related to developing a research hypothesis or referring to a specific type of hypothesis. Let's take a quick look at these terms.

Null hypothesis

The term null hypothesis refers to a research hypothesis type that assumes no statistically significant relationship exists within a set of observations or data. It represents a claim that assumes that any observed relationship is due to chance. Represented as H0, the null represents the conjecture of the research.

Alternative hypothesis

The alternative hypothesis accompanies the null hypothesis. It states that the situation presented in the null hypothesis is false or untrue, and claims an observed effect in your test. This is typically denoted by Ha or H(n), where “n” stands for the number of alternative hypotheses. You can have more than one alternative hypothesis. 

Simple hypothesis

The term simple hypothesis refers to a hypothesis or theory that predicts the relationship between two variables - the independent (predictor) and the dependent (predicted). 

Complex hypothesis

The term complex hypothesis refers to a model – either quantitative (mathematical) or qualitative . A complex hypothesis states the surmised relationship between two or more potentially related variables.

Directional hypothesis

When creating a statistical hypothesis, the directional hypothesis (the null hypothesis) states an assumption regarding one parameter of a population. Some academics call this the “one-sided” hypothesis. The alternative hypothesis indicates whether the researcher tests for a positive or negative effect by including either the greater than (">") or less than ("<") sign.

Non-directional hypothesis

We refer to the alternative hypothesis in a statistical research question as a non-directional hypothesis. It includes the not equal ("≠") sign to show that the research tests whether or not an effect exists without specifying the effect's direction (positive or negative).

Associative hypothesis

The term associative hypothesis assumes a link between two variables but stops short of stating that one variable impacts the other. Academic statistical literature asserts in this sense that correlation does not imply causation. So, although the hypothesis notes the correlation between two variables – the independent and dependent - it does not predict how the two interact.

Logical hypothesis

Typically used in philosophy rather than science, researchers can't test a logical hypothesis because the technology or data set doesn't yet exist. A logical hypothesis uses logic as the basis of its assumptions. 

In some cases, a logical hypothesis can become an empirical hypothesis once technology provides an opportunity for testing. Until that time, the question remains too expensive or complex to address. Note that a logical hypothesis is not a statistical hypothesis.

Empirical hypothesis

When we consider the opposite of a logical hypothesis, we call this an empirical or working hypothesis. This type of hypothesis considers a scientifically measurable question. A researcher can consider and test an empirical hypothesis through replicable tests, observations, and measurements.

Statistical hypothesis

The term statistical hypothesis refers to a test of a theory that uses representative statistical models to test relationships between variables to draw conclusions regarding a large population. This requires an existing large data set, commonly referred to as big data, or implementing a survey to obtain original statistical information to form a data set for the study. 

Testing this type of hypothesis requires the use of random samples. Note that the null and alternative hypotheses are used in statistical hypothesis testing.

Causal hypothesis

The term causal hypothesis refers to a research hypothesis that tests a cause-and-effect relationship. A causal hypothesis is utilized when conducting experimental or quasi-experimental research.

Descriptive hypothesis

The term descriptive hypothesis refers to a research hypothesis used in non-experimental research, specifying an influence in the relationship between two variables.

• What makes an effective research hypothesis?

An effective research hypothesis offers a clearly defined, specific statement, using simple wording that contains no assumptions or generalizations, and that you can test. A well-written hypothesis should predict the tested relationship and its outcome. It contains zero ambiguity and offers results you can observe and test. 

The research hypothesis should address a question relevant to a research area. Overall, your research hypothesis needs the following essentials:

Hypothesis Essential #1: Specificity & Clarity

Hypothesis Essential #2: Testability (Provability)

• How to develop a good research hypothesis

In developing your hypothesis statements, you must pre-plan some of your statistical analysis. Once you decide on your problem to examine, determine three aspects:

the parameter you'll test

the test's direction (left-tailed, right-tailed, or non-directional)

the hypothesized parameter value

Any quantitative research includes a hypothesized parameter value of a mean, a proportion, or the difference between two proportions. Here's how to note each parameter:

Single mean (μ)

Paired means (μd)

Single proportion (p)

Difference between two independent means (μ1−μ2)

Difference between two proportions (p1−p2)

Simple linear regression slope (β)

Correlation (ρ)

Defining these parameters and determining whether you want to test the mean, proportion, or differences helps you determine the statistical tests you'll conduct to analyze your data. When writing your hypothesis, you only need to decide which parameter to test and in what overarching way.

The null research hypothesis must include everyday language, in a single sentence, stating the problem you want to solve. Write it as an if-then statement with defined variables. Write an alternative research hypothesis that states the opposite.

• What is the correct format for writing a hypothesis?

The following example shows the proper format and textual content of a hypothesis. It follows commonly accepted academic standards.

Null hypothesis (H0): High school students who participate in varsity sports as opposed to those who do not, fail to score higher on leadership tests than students who do not participate.

Alternative hypothesis (H1): High school students who play a varsity sport as opposed to those who do not participate in team athletics will score higher on leadership tests than students who do not participate in athletics.

The research question tests the correlation between varsity sports participation and leadership qualities expressed as a score on leadership tests. It compares the population of athletes to non-athletes.

• What are the five steps of a hypothesis?

Once you decide on the specific problem or question you want to address, you can write your research hypothesis. Use this five-step system to hone your null hypothesis and generate your alternative hypothesis.

Step 1 : Create your research question. This topic should interest and excite you; answering it provides relevant information to an industry or academic area.

Step 2 : Conduct a literature review to gather essential existing research.

Step 3 : Write a clear, strong, simply worded sentence that explains your test parameter, test direction, and hypothesized parameter.

Step 4 : Read it a few times. Have others read it and ask them what they think it means. Refine your statement accordingly until it becomes understandable to everyone. While not everyone can or will comprehend every research study conducted, any person from the general population should be able to read your hypothesis and alternative hypothesis and understand the essential question you want to answer.

Step 5 : Re-write your null hypothesis until it reads simply and understandably. Write your alternative hypothesis.

What is the Red Queen hypothesis?

Some hypotheses are well-known, such as the Red Queen hypothesis. Choose your wording carefully, since you could become like the famed scientist Dr. Leigh Van Valen. In 1973, Dr. Van Valen proposed the Red Queen hypothesis to describe coevolutionary activity, specifically reciprocal evolutionary effects between species to explain extinction rates in the fossil record. 

Essentially, Van Valen theorized that to survive, each species remains in a constant state of adaptation, evolution, and proliferation, and constantly competes for survival alongside other species doing the same. Only by doing this can a species avoid extinction. Van Valen took the hypothesis title from the Lewis Carroll book, "Through the Looking Glass," which contains a key character named the Red Queen who explains to Alice that for all of her running, she's merely running in place.

• Getting started with your research

In conclusion, once you write your null hypothesis (H0) and an alternative hypothesis (Ha), you’ve essentially authored the elevator pitch of your research. These two one-sentence statements describe your topic in simple, understandable terms that both professionals and laymen can understand. They provide the starting point of your research project.

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Research Hypothesis In Psychology: Types, & Examples

Saul McLeod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul McLeod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

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A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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A Beginner’s Guide to Hypothesis Testing in Business

• 30 Mar 2021

Becoming a more data-driven decision-maker can bring several benefits to your organization, enabling you to identify new opportunities to pursue and threats to abate. Rather than allowing subjective thinking to guide your business strategy, backing your decisions with data can empower your company to become more innovative and, ultimately, profitable.

If you’re new to data-driven decision-making, you might be wondering how data translates into business strategy. The answer lies in generating a hypothesis and verifying or rejecting it based on what various forms of data tell you.

Below is a look at hypothesis testing and the role it plays in helping businesses become more data-driven.

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What Is Hypothesis Testing?

To understand what hypothesis testing is, it’s important first to understand what a hypothesis is.

A hypothesis or hypothesis statement seeks to explain why something has happened, or what might happen, under certain conditions. It can also be used to understand how different variables relate to each other. Hypotheses are often written as if-then statements; for example, “If this happens, then this will happen.”

Hypothesis testing , then, is a statistical means of testing an assumption stated in a hypothesis. While the specific methodology leveraged depends on the nature of the hypothesis and data available, hypothesis testing typically uses sample data to extrapolate insights about a larger population.

Hypothesis Testing in Business

When it comes to data-driven decision-making, there’s a certain amount of risk that can mislead a professional. This could be due to flawed thinking or observations, incomplete or inaccurate data , or the presence of unknown variables. The danger in this is that, if major strategic decisions are made based on flawed insights, it can lead to wasted resources, missed opportunities, and catastrophic outcomes.

The real value of hypothesis testing in business is that it allows professionals to test their theories and assumptions before putting them into action. This essentially allows an organization to verify its analysis is correct before committing resources to implement a broader strategy.

As one example, consider a company that wishes to launch a new marketing campaign to revitalize sales during a slow period. Doing so could be an incredibly expensive endeavor, depending on the campaign’s size and complexity. The company, therefore, may wish to test the campaign on a smaller scale to understand how it will perform.

In this example, the hypothesis that’s being tested would fall along the lines of: “If the company launches a new marketing campaign, then it will translate into an increase in sales.” It may even be possible to quantify how much of a lift in sales the company expects to see from the effort. Pending the results of the pilot campaign, the business would then know whether it makes sense to roll it out more broadly.

Related: 9 Fundamental Data Science Skills for Business Professionals

Key Considerations for Hypothesis Testing

1. alternative hypothesis and null hypothesis.

In hypothesis testing, the hypothesis that’s being tested is known as the alternative hypothesis . Often, it’s expressed as a correlation or statistical relationship between variables. The null hypothesis , on the other hand, is a statement that’s meant to show there’s no statistical relationship between the variables being tested. It’s typically the exact opposite of whatever is stated in the alternative hypothesis.

For example, consider a company’s leadership team that historically and reliably sees $12 million in monthly revenue. They want to understand if reducing the price of their services will attract more customers and, in turn, increase revenue.

In this case, the alternative hypothesis may take the form of a statement such as: “If we reduce the price of our flagship service by five percent, then we’ll see an increase in sales and realize revenues greater than $12 million in the next month.”

The null hypothesis, on the other hand, would indicate that revenues wouldn’t increase from the base of $12 million, or might even decrease.

Check out the video below about the difference between an alternative and a null hypothesis, and subscribe to our YouTube channel for more explainer content.

2. Significance Level and P-Value

Statistically speaking, if you were to run the same scenario 100 times, you’d likely receive somewhat different results each time. If you were to plot these results in a distribution plot, you’d see the most likely outcome is at the tallest point in the graph, with less likely outcomes falling to the right and left of that point.

With this in mind, imagine you’ve completed your hypothesis test and have your results, which indicate there may be a correlation between the variables you were testing. To understand your results' significance, you’ll need to identify a p-value for the test, which helps note how confident you are in the test results.

In statistics, the p-value depicts the probability that, assuming the null hypothesis is correct, you might still observe results that are at least as extreme as the results of your hypothesis test. The smaller the p-value, the more likely the alternative hypothesis is correct, and the greater the significance of your results.

3. One-Sided vs. Two-Sided Testing

When it’s time to test your hypothesis, it’s important to leverage the correct testing method. The two most common hypothesis testing methods are one-sided and two-sided tests , or one-tailed and two-tailed tests, respectively.

Typically, you’d leverage a one-sided test when you have a strong conviction about the direction of change you expect to see due to your hypothesis test. You’d leverage a two-sided test when you’re less confident in the direction of change.

4. Sampling

To perform hypothesis testing in the first place, you need to collect a sample of data to be analyzed. Depending on the question you’re seeking to answer or investigate, you might collect samples through surveys, observational studies, or experiments.

A survey involves asking a series of questions to a random population sample and recording self-reported responses.

Observational studies involve a researcher observing a sample population and collecting data as it occurs naturally, without intervention.

Finally, an experiment involves dividing a sample into multiple groups, one of which acts as the control group. For each non-control group, the variable being studied is manipulated to determine how the data collected differs from that of the control group.

Learn How to Perform Hypothesis Testing

Hypothesis testing is a complex process involving different moving pieces that can allow an organization to effectively leverage its data and inform strategic decisions.

If you’re interested in better understanding hypothesis testing and the role it can play within your organization, one option is to complete a course that focuses on the process. Doing so can lay the statistical and analytical foundation you need to succeed.

Do you want to learn more about hypothesis testing? Explore Business Analytics —one of our online business essentials courses —and download our Beginner’s Guide to Data & Analytics .

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• Developing and Testing Hypotheses

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Statistical Analysis: Developing and Testing Hypotheses

Statistical hypothesis testing is sometimes known as confirmatory data analysis. It is a way of drawing inferences from data. In the process, you develop a hypothesis or theory about what you might see in your research. You then test that hypothesis against the data that you collect.

Hypothesis testing is generally used when you want to compare two groups, or compare a group against an idealised position.

Before You Start: Developing A Research Hypothesis

Before you can do any kind of research in social science fields such as management, you need a research question or hypothesis. Research is generally designed to either answer a research question or consider a research hypothesis . These two are closely linked, and generally one or the other is used, rather than both.

A research question is the question that your research sets out to answer . For example:

Do men and women like ice cream equally?

Do men and women like the same flavours of ice cream?

What are the main problems in the market for ice cream?

How can the market for ice cream be segmented and targeted?

Research hypotheses are statements of what you believe you will find in your research.

These are then tested statistically during the research to see if your belief is correct. Examples include:

Men and women like ice cream to different extents.

Men and women like different flavours of ice cream.

Men are more likely than women to like mint ice cream.

Women are more likely than men to like chocolate ice cream.

Both men and women prefer strawberry to vanilla ice cream.

Relationships vs Differences

Research hypotheses can be expressed in terms of differences between groups, or relationships between variables. However, these are two sides of the same coin: almost any hypothesis could be set out in either way.

For example:

There is a relationship between gender and liking ice cream OR

Men are more likely to like ice cream than women.

Testing Research Hypotheses

The purpose of statistical hypothesis testing is to use a sample to draw inferences about a population.

Testing research hypotheses requires a number of steps:

Step 1. Define your research hypothesis

The first step in any hypothesis testing is to identify your hypothesis, which you will then go on to test. How you define your hypothesis may affect the type of statistical testing that you do, so it is important to be clear about it. In particular, consider whether you are going to hypothesise simply that there is a relationship or speculate about the direction of the relationship.

Using the examples above:

There is a relationship between gender and liking ice cream is a non-directional hypothesis. You have simply specified that there is a relationship, not whether men or women like ice cream more.

However, men are more likely to like ice cream than women is directional : you have specified which gender is more likely to like ice cream.

Generally, it is better not to specify direction unless you are moderately sure about it.

Step 2. Define the null hypothesis

The null hypothesis is basically a statement of what you are hoping to disprove: the opposite of your ‘guess’ about the relationship. For example, in the hypotheses above, the null hypothesis would be:

Men and women like ice cream equally, or

There is no relationship between gender and ice cream.

This also defines your ‘alternative hypothesis’ which is your ‘test hypothesis’ ( men like ice cream more than women ). Your null hypothesis is generally that there is no difference, because this is the simplest position.

The purpose of hypothesis testing is to disprove the null hypothesis. If you cannot disprove the null hypothesis, you have to assume it is correct.

Step 3. Develop a summary measure that describes your variable of interest for each group you wish to compare

Our page on Simple Statistical Analysis describes several summary measures, including two of the most common, mean and median.

The next step in your hypothesis testing is to develop a summary measure for each of your groups. For example, to test the gender differences in liking for ice cream, you might ask people how much they liked ice cream on a scale of 1 to 5. Alternatively, you might have data about the number of times that ice creams are consumed each week in the summer months.

You then need to produce a summary measure for each group, usually mean and standard deviation. These may be similar for each group, or quite different.

Step 4. Choose a reference distribution and calculate a test statistic

To decide whether there is a genuine difference between the two groups, you have to use a reference distribution against which to measure the values from the two groups.

The most common source of reference distributions is a standard distribution such as the normal distribution or t - distribution. These two are the same, except that the standard deviation of the t -distribution is estimated from the sample, and that of the normal distribution is known. There is more about this in our page on Statistical Distributions .

You then compare the summary data from the two groups by using them to calculate a test statistic. There is a standard formula for every test statistic and reference distribution. The test and reference distribution depend on your data and the purpose of your testing (see below).

The test that you use to compare your groups will depend on how many groups you have, the type of data that you have collected, and how reliable your data are. In general, you would use different tests for comparing two groups than you would for comparing three or more.

Our page Surveys and Survey Design explains that there are two types of answer scale, continuous and categorical. Age, for example, is a continuous scale, although it can also be grouped into categories. You may also find it helpful to read our page on Types of Data .

Gender is a category scale.

For a continuous scale, you can use the mean values of the two groups that you are comparing.

For a category scale, you need to use the median values.

Source: Easterby-Smith, Thorpe and Jackson, Management Research 4th Edition

One- or Two-Tailed Test

The other thing that you have to decide is whether you use what is known as a ‘one-tailed’ or ‘two-tailed’ test.

This allows you to compare differences between groups in either one or both directions.

In practice, this boils down to whether your research hypothesis is expressed as ‘x is likely to be more than y’, or ‘x is likely to be different from y’. If you are confident of the direction of the distance (that is, you are sure that the only options are that ‘x is likely to be more than y’ or ‘x and y are the same’), then your test will be one-tailed. If not, it will be two-tailed .

If there is any doubt, it is better to use a two-tailed test.

You should only use a one-tailed test when you are certain about the direction of the difference, and it doesn’t matter if you are wrong.

The graph under Step 5 shows a two-tailed test.

If you are not very confident about the quality of the data collected, for example because the inputting was done quickly and cheaply, or because the data have not been checked, then you may prefer to use the median  even if the data are continuous  to avoid any problems with outliers. This makes the tests more robust, and the results more reliable.

Our page on correlations suggests that you may also want to plot a scattergraph before undertaking any further analysis. This will also help you to identify any outliers or potential problems with the data.

Calculating the Test Statistic

For each type of test, there is a standard formula for the test statistic. For example, for the t -test, it is:

(M1-M2)/SE(diff)

M1 is the mean of the first group

M2 is the mean of the second group

SE(diff) is the standard error of the difference, which is calculated from the standard deviation and the sample size of each group.

The formula for calculating the standard error of the difference between means is:

• sd 2 = the square of the standard deviation of the source population (i.e., the variance);
• n a = the size of sample A; and
• n b = the size of sample B.

Step 5. Identify Acceptance and Rejection Regions

The final part of the test is to see if your test statistic is significant—in other words, whether you are going to accept or reject your null hypothesis. You need to consider first what level of significance is required. This tells you the probability that you have achieved your result by chance.

Significance (or p-value) is usually required to be either 5% or 1%, meaning that you are 95% or 99% confident that your result was not achieved by chance.

NOTE:  the significance level is sometimes expressed as  p  < 0.05 or  p  < 0.01.

For more about significance, you may like to read our page on Significance and Confidence Intervals .

The graph below shows a reference distribution (this one could be either the normal or the t- distribution) with the acceptance and rejection regions marked. It also shows the critical values. µ is the mean. For more about this, you may like to read our page on Statistical Distributions .

The critical values are identified from published statistical tables for your reference distribution, which are available for different levels of significance.

If your test statistic falls within either of the two rejection regions (that is, it is greater than the higher critical value, or less than the lower one), you will reject the null hypothesis. You can therefore accept your alternative hypothesis.

Step 6. Draw Conclusions and Inferences

The final step is to draw conclusions.

If your test statistic fell within the rejection region, and you have rejected the null hypothesis, you can therefore conclude that there is a gender difference in liking for ice cream, using the example above.

Types of Error

There are four possible outcomes from statistical testing (see table):

The groups are different, and you conclude that they are different (correct result)

The groups are different, but you conclude that they are not (Type II error)

The groups are the same, but you conclude that they are different (Type I error)

The groups are the same, and you conclude that they are the same (correct result).

Type I errors are generally considered more important than Type II, because they have the potential to change the status quo.

For example, if you wrongly conclude that a new medical treatment is effective, doctors are likely to move to providing that treatment. Patients may receive the treatment instead of an alternative that could have fewer side effects, and pharmaceutical companies may stop looking for an alternative treatment.

Further Reading from Skills You Need

Data Handling and Algebra Part of The Skills You Need Guide to Numeracy

This eBook covers the basics of data handling, data visualisation, basic statistical analysis and algebra. The book contains plenty of worked examples to improve understanding as well as real-world examples to show you how these concepts are useful.

Whether you want to brush up on your basics, or help your children with their learning, this is the book for you.

There are statistical software packages available that will carry out all these tests for you. However, if you have never studied statistics, and you’re not very confident about what you’re doing, you are probably best off discussing it with a statistician or consulting a detailed statistical textbook.

Poorly executed statistical analysis can invalidate very good research.  It is much better to find someone to help you. However, this page will help you to understand your friendly statistician!

Continue to: Significance and Confidence Intervals Statistical Analysis: Types of Data

See also: Understanding Correlations Understanding Statistical Distributions Averages (Mean, Median and Mode)

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

About the author

Muhammad Hassan

Researcher, Academic Writer, Web developer

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• > Machine Learning
• > Statistics

What is Hypothesis Testing? Types and Methods

• Soumyaa Rawat
• Jul 23, 2021

Hypothesis Testing  

Hypothesis testing is the act of testing a hypothesis or a supposition in relation to a statistical parameter. Analysts implement hypothesis testing in order to test if a hypothesis is plausible or not. 

In data science and statistics , hypothesis testing is an important step as it involves the verification of an assumption that could help develop a statistical parameter. For instance, a researcher establishes a hypothesis assuming that the average of all odd numbers is an even number. 

In order to find the plausibility of this hypothesis, the researcher will have to test the hypothesis using hypothesis testing methods. Unlike a hypothesis that is ‘supposed’ to stand true on the basis of little or no evidence, hypothesis testing is required to have plausible evidence in order to establish that a statistical hypothesis is true. 

Perhaps this is where statistics play an important role. A number of components are involved in this process. But before understanding the process involved in hypothesis testing in research methodology, we shall first understand the types of hypotheses that are involved in the process. Let us get started! 

Types of Hypotheses

In data sampling, different types of hypothesis are involved in finding whether the tested samples test positive for a hypothesis or not. In this segment, we shall discover the different types of hypotheses and understand the role they play in hypothesis testing.

Alternative Hypothesis

Alternative Hypothesis (H1) or the research hypothesis states that there is a relationship between two variables (where one variable affects the other). The alternative hypothesis is the main driving force for hypothesis testing. 

It implies that the two variables are related to each other and the relationship that exists between them is not due to chance or coincidence. 

When the process of hypothesis testing is carried out, the alternative hypothesis is the main subject of the testing process. The analyst intends to test the alternative hypothesis and verifies its plausibility.

Null Hypothesis

The Null Hypothesis (H0) aims to nullify the alternative hypothesis by implying that there exists no relation between two variables in statistics. It states that the effect of one variable on the other is solely due to chance and no empirical cause lies behind it. 

The null hypothesis is established alongside the alternative hypothesis and is recognized as important as the latter. In hypothesis testing, the null hypothesis has a major role to play as it influences the testing against the alternative hypothesis. 

(Must read: What is ANOVA test? )

Non-Directional Hypothesis

The Non-directional hypothesis states that the relation between two variables has no direction. 

Simply put, it asserts that there exists a relation between two variables, but does not recognize the direction of effect, whether variable A affects variable B or vice versa. 

Directional Hypothesis

The Directional hypothesis, on the other hand, asserts the direction of effect of the relationship that exists between two variables. 

Herein, the hypothesis clearly states that variable A affects variable B, or vice versa. 

Statistical Hypothesis

A statistical hypothesis is a hypothesis that can be verified to be plausible on the basis of statistics. 

By using data sampling and statistical knowledge, one can determine the plausibility of a statistical hypothesis and find out if it stands true or not. 

(Related blog: z-test vs t-test )

Performing Hypothesis Testing  

Now that we have understood the types of hypotheses and the role they play in hypothesis testing, let us now move on to understand the process in a better manner. 

In hypothesis testing, a researcher is first required to establish two hypotheses - alternative hypothesis and null hypothesis in order to begin with the procedure. 

To establish these two hypotheses, one is required to study data samples, find a plausible pattern among the samples, and pen down a statistical hypothesis that they wish to test. 

A random population of samples can be drawn, to begin with hypothesis testing. Among the two hypotheses, alternative and null, only one can be verified to be true. Perhaps the presence of both hypotheses is required to make the process successful. 

At the end of the hypothesis testing procedure, either of the hypotheses will be rejected and the other one will be supported. Even though one of the two hypotheses turns out to be true, no hypothesis can ever be verified 100%. 

(Read also: Types of data sampling techniques )

Therefore, a hypothesis can only be supported based on the statistical samples and verified data. Here is a step-by-step guide for hypothesis testing.

Establish the hypotheses

First things first, one is required to establish two hypotheses - alternative and null, that will set the foundation for hypothesis testing. 

These hypotheses initiate the testing process that involves the researcher working on data samples in order to either support the alternative hypothesis or the null hypothesis. 

Generate a testing plan

Once the hypotheses have been formulated, it is now time to generate a testing plan. A testing plan or an analysis plan involves the accumulation of data samples, determining which statistic is to be considered and laying out the sample size. 

All these factors are very important while one is working on hypothesis testing.

Analyze data samples

As soon as a testing plan is ready, it is time to move on to the analysis part. Analysis of data samples involves configuring statistical values of samples, drawing them together, and deriving a pattern out of these samples. 

While analyzing the data samples, a researcher needs to determine a set of things -

Significance Level - The level of significance in hypothesis testing indicates if a statistical result could have significance if the null hypothesis stands to be true.

Testing Method - The testing method involves a type of sampling-distribution and a test statistic that leads to hypothesis testing. There are a number of testing methods that can assist in the analysis of data samples. 

Test statistic - Test statistic is a numerical summary of a data set that can be used to perform hypothesis testing.

P-value - The P-value interpretation is the probability of finding a sample statistic to be as extreme as the test statistic, indicating the plausibility of the null hypothesis. 

Infer the results

The analysis of data samples leads to the inference of results that establishes whether the alternative hypothesis stands true or not. When the P-value is less than the significance level, the null hypothesis is rejected and the alternative hypothesis turns out to be plausible. 

Methods of Hypothesis Testing

As we have already looked into different aspects of hypothesis testing, we shall now look into the different methods of hypothesis testing. All in all, there are 2 most common types of hypothesis testing methods. They are as follows -

Frequentist Hypothesis Testing

The frequentist hypothesis or the traditional approach to hypothesis testing is a hypothesis testing method that aims on making assumptions by considering current data. 

The supposed truths and assumptions are based on the current data and a set of 2 hypotheses are formulated. A very popular subtype of the frequentist approach is the Null Hypothesis Significance Testing (NHST). 

The NHST approach (involving the null and alternative hypothesis) has been one of the most sought-after methods of hypothesis testing in the field of statistics ever since its inception in the mid-1950s. 

Bayesian Hypothesis Testing

A much unconventional and modern method of hypothesis testing, the Bayesian Hypothesis Testing claims to test a particular hypothesis in accordance with the past data samples, known as prior probability, and current data that lead to the plausibility of a hypothesis. 

The result obtained indicates the posterior probability of the hypothesis. In this method, the researcher relies on ‘prior probability and posterior probability’ to conduct hypothesis testing on hand. 

On the basis of this prior probability, the Bayesian approach tests a hypothesis to be true or false. The Bayes factor, a major component of this method, indicates the likelihood ratio among the null hypothesis and the alternative hypothesis. 

The Bayes factor is the indicator of the plausibility of either of the two hypotheses that are established for hypothesis testing.  

(Also read - Introduction to Bayesian Statistics ) 

To conclude, hypothesis testing, a way to verify the plausibility of a supposed assumption can be done through different methods - the Bayesian approach or the Frequentist approach. 

Although the Bayesian approach relies on the prior probability of data samples, the frequentist approach assumes without a probability. A number of elements involved in hypothesis testing are - significance level, p-level, test statistic, and method of hypothesis testing. 

(Also read: Introduction to probability distributions )

A significant way to determine whether a hypothesis stands true or not is to verify the data samples and identify the plausible hypothesis among the null hypothesis and alternative hypothesis. 

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Mar 21, 2024

Creating a research hypothesis: How to formulate and test UX expectations

A research hypothesis helps guide your UX research with focused predictions you can test and learn from. Here’s how to formulate your own hypotheses.

Armin Tanovic

All great products were once just thoughts—the spark of an idea waiting to be turned into something tangible.

A research hypothesis in UX is very similar. It’s the starting point for your user research; the jumping off point for your product development initiatives.

Formulating a UX research hypothesis helps guide your UX research project in the right direction, collect insights, and evaluate not only whether an idea is worth pursuing, but how to go after it.

In this article, we’ll cover what a research hypothesis is, how it's relevant to UX research, and the best formula to create your own hypothesis and put it to the test.

Test your hypothesis with Maze

Maze lets you validate your design and test research hypotheses to move forward with authentic user insights.

What defines a research hypothesis?

A research hypothesis is a statement or prediction that needs testing to be proven or disproven.

Let’s say you’ve got an inkling that making a change to a feature icon will increase the number of users that engage with it—with some minor adjustments, this theory becomes a research hypothesis: “ Adjusting Feature X’s icon will increase daily average users by 20% ”.

A research hypothesis is the starting point that guides user research . It takes your thought and turns it into something you can quantify and evaluate. In this case, you could conduct usability tests and user surveys, and run A/B tests to see if you’re right—or, just as importantly, wrong .

A good research hypothesis has three main features:

• Specificity: A hypothesis should clearly define what variables you’re studying and what you expect an outcome to be, without ambiguity in its wording
• Relevance: A research hypothesis should have significance for your research project by addressing a potential opportunity for improvement
• Testability: Your research hypothesis must be able to be tested in some way such as empirical observation or data collection

What is the difference between a research hypothesis and a research question?

Research questions and research hypotheses are often treated as one and the same, but they’re not quite identical.

A research hypothesis acts as a prediction or educated guess of outcomes , while a research question poses a query on the subject you’re investigating. Put simply, a research hypothesis is a statement, whereas a research question is (you guessed it) a question.

For example, here’s a research hypothesis: “ Implementing a navigation bar on our dashboard will improve customer satisfaction scores by 10%. ”

This statement acts as a testable prediction. It doesn’t pose a question, it’s a prediction. Here’s what the same hypothesis would look like as a research question: “ Will integrating a navigation bar on our dashboard improve customer satisfaction scores? ”

The distinction is minor, and both are focused on uncovering the truth behind the topic, but they’re not quite the same.

Why do you use a research hypothesis in UX?

Research hypotheses in UX are used to establish the direction of a particular study, research project, or test. Formulating a hypothesis and testing it ensures the UX research you conduct is methodical, focused, and actionable. It aids every phase of your research process , acting as a north star that guides your efforts toward successful product development .

Typically, UX researchers will formulate a testable hypothesis to help them fulfill a broader objective, such as improving customer experience or product usability. They’ll then conduct user research to gain insights into their prediction and confirm or reject the hypothesis.

A proven or disproven hypothesis will tell if your prediction is right, and whether you should move forward with your proposed design—or if it's back to the drawing board.

Formulating a hypothesis can be helpful in anything from prototype testing to idea validation, and design iteration. Put simply, it’s one of the first steps in conducting user research.

Whether you’re in the initial stages of product discovery for a new product, a single feature, or conducting ongoing research, a strong hypothesis presents a clear purpose and angle for your research It also helps understand which user research methodology to use to get your answers.

What are the types of research hypotheses?

Not all hypotheses are built the same—there are different types with different objectives. Understanding the different types enables you to formulate a research hypothesis that outlines the angle you need to take to prove or disprove your predictions.

Here are some of the different types of hypotheses to keep in mind.

Null and alternative hypotheses

While a normal research hypothesis predicts that a specific outcome will occur based upon a certain change of variables, a null hypothesis predicts that no difference will occur when you introduce a new condition.

By that reasoning, a null hypothesis would be:

• Adding a new CTA button to the top of our homepage will make no difference in conversions

Null hypotheses are useful because they help outline what your test or research study is trying to dis prove, rather than prove, through a research hypothesis.

An alternative hypothesis states the exact opposite of a null hypothesis. It proposes that a certain change will occur when you introduce a new condition or variable. For example:

• Adding a CTA button to the top of our homepage will cause a difference in conversion rates

Simple hypotheses and complex hypotheses

A simple hypothesis is a prediction that includes only two variables in a cause-and-effect sequence, with one variable dependent on the other. It predicts that you'll achieve a particular outcome based on a certain condition. The outcome is known as the dependent variable and the change causing it is the independent variable .

For example, this is a simple hypothesis:

• Including the search function on our mobile app will increase user retention

The expected outcome of increasing user retention is based on the condition of including a new search function. But, what happens when there are more than two factors at play?

We get what’s called a complex hypothesis. Instead of a simple condition and outcome, complex hypotheses include multiple results. This makes them a perfect research hypothesis type for framing complex studies or tracking multiple KPIs based on a single action.

Building upon our previous example, a complex research hypothesis could be:

• Including the search function on our mobile app will increase user retention and boost conversions

Directional and non-directional hypotheses

Research hypotheses can also differ in the specificity of outcomes. Put simply, any hypothesis that has a specific outcome or direction based on the relationship of its variables is a directional hypothesis . That means that our previous example of a simple hypothesis is also a directional hypothesis.

Non-directional hypotheses don’t specify the outcome or difference the variables will see. They just state that a difference exists. Following our example above, here’s what a non-directional hypothesis would look like:

• Including the search function on our mobile app will make a difference in user retention

In this non-directional hypothesis, the direction of difference (increase/decrease) hasn’t been specified, we’ve just noted that there will be a difference.

The type of hypothesis you write helps guide your research—let’s get into it.

How to write and test your UX research hypothesis

Now we’ve covered the types of research hypothesis examples, it’s time to get practical.

Creating your research hypothesis is the first step in conducting successful user research.

Here are the four steps for writing and testing a UX research hypothesis to help you make informed, data-backed decisions for product design and development.

1. Formulate your hypothesis

Start by writing out your hypothesis in a way that’s specific and relevant to a distinct aspect of your user or product experience. Meaning: your prediction should include a design choice followed by the outcome you’d expect—this is what you’re looking to validate or reject.

Your proposed research hypothesis should also be testable through user research data analysis. There’s little point in a hypothesis you can’t test!

Let’s say your focus is your product’s user interface—and how you can improve it to better meet customer needs. A research hypothesis in this instance might be:

• Adding a settings tab to the navigation bar will improve usability

By writing out a research hypothesis in this way, you’re able to conduct relevant user research to prove or disprove your hypothesis. You can then use the results of your research—and the validation or rejection of your hypothesis—to decide whether or not you need to make changes to your product’s interface.

2. Identify variables and choose your research method

Once you’ve got your hypothesis, you need to map out how exactly you’ll test it. Consider what variables relate to your hypothesis. In our case, the main variable of our outcome is adding a settings tab to the navigation bar.

Once you’ve defined the relevant variables, you’re in a better position to decide on the best UX research method for the job. If you’re after metrics that signal improvement, you’ll want to select a method yielding quantifiable results—like usability testing . If your outcome is geared toward what users feel, then research methods for qualitative user insights, like user interviews , are the way to go.

3. Carry out your study

It’s go time. Now you’ve got your hypothesis, identified the relevant variables, and outlined your method for testing them, you’re ready to run your study. This step involves recruiting participants for your study and reaching out to them through relevant channels like email, live website testing , or social media.

Given our hypothesis, our best bet is to conduct A/B and usability tests with a prototype that includes the additional UI elements, then compare the usability metrics to see whether users find navigation easier with or without the settings button.

We can also follow up with UX surveys to get qualitative insights and ask users how they found the task, what they preferred about each design, and to see what additional customer insights we uncover.

💡 Want more insights from your usability tests? Maze Clips enables you to gather real-time recordings and reactions of users participating in usability tests .

4. Analyze your results and compare them to your hypothesis

By this point, you’ve neatly outlined a hypothesis, chosen a research method, and carried out your study. It’s now time to analyze your findings and evaluate whether they support or reject your hypothesis.

Look at the data you’ve collected and what it means. Given that we conducted usability testing, we’ll want to look to some key usability metrics for an indication of whether the additional settings button improves usability.

For example, with the usability task of ‘ In account settings, find your profile and change your username ’, we can conduct task analysis to compare the times spent on task and misclick rates of the new design, with those same metrics from the old design.

If you also conduct follow-up surveys or interviews, you can ask users directly about their experience and analyze their answers to gather additional qualitative data . Maze AI can handle the analysis automatically, but you can also manually read through responses to get an idea of what users think about the change.

By comparing the findings to your research hypothesis, you can identify whether your research accepts or rejects your hypothesis. If the majority of users struggle with finding the settings page within usability tests, but had a higher success rate with your new prototype, you’ve proved the hypothesis.

However, it's also crucial to acknowledge if the findings refute your hypothesis rather than prove it as true. Ruling something out is just as valuable as confirming a suspicion.

In either case, make sure to draw conclusions based on the relationship between the variables and store findings in your UX research repository . You can conduct deeper analysis with techniques like thematic analysis or affinity mapping .

UX research hypotheses: four best practices to guide your research

Knowing the big steps for formulating and testing a research hypothesis ensures that your next UX research project gives you focused, impactful results and insights. But, that’s only the tip of the research hypothesis iceberg. There are some best practices you’ll want to consider when using a hypothesis to test your UX design ideas.

Here are four research hypothesis best practices to help guide testing and make your UX research systematic and actionable.

Align your hypothesis to broader business and UX goals

Before you begin to formulate your hypothesis, be sure to pause and think about how it connects to broader goals in your UX strategy . This ensures that your efforts and predictions align with your overarching design and development goals.

For example, implementing a brand new navigation menu for current account holders might work for usability, but if the wider team is focused on boosting conversion rates for first-time site viewers, there might be a different research project to prioritize.

Create clear and actionable reports for stakeholders

Once you’ve conducted your testing and proved or disproved your hypothesis, UX reporting and analysis is the next step. You’ll need to present your findings to stakeholders in a way that's clear, concise, and actionable. If your hypothesis insights come in the form of metrics and statistics, then quantitative data visualization tools and reports will help stakeholders understand the significance of your study, while setting the stage for design changes and solutions.

If you went with a research method like user interviews, a narrative UX research report including key themes and findings, proposed solutions, and your original hypothesis will help inform your stakeholders on the best course of action.

Consider different user segments

While getting enough responses is crucial for proving or disproving your hypothesis, you’ll want to consider which users will give you the highest quality and most relevant responses. Remember to consider user personas —e.g. If you’re only introducing a change for premium users, exclude testing with users who are on a free trial of your product.

You can recruit and target specific user demographics with the Maze Panel —which enables you to search for and filter participants that meet your requirements. Doing so allows you to better understand how different users will respond to your hypothesis testing. It also helps you uncover specific needs or issues different users may have.

Involve stakeholders from the start

Before testing or even formulating a research hypothesis by yourself, ensure all your stakeholders are on board. Informing everyone of your plan to formulate and test your hypothesis does three things:

Firstly, it keeps your team in the loop . They’ll be able to inform you of any relevant insights, special considerations, or existing data they already have about your particular design change idea, or KPIs to consider that would benefit the wider team.

Secondly, informing stakeholders ensures seamless collaboration across multiple departments . Together, you’ll be able to fit your testing results into your overall CX strategy , ensuring alignment with business goals and broader objectives.

Finally, getting everyone involved enables them to contribute potential hypotheses to test . You’re not the only one with ideas about what changes could positively impact the user experience, and keeping everyone in the loop brings fresh ideas and perspectives to the table.

Test your UX research hypotheses with Maze

Formulating and testing out a research hypothesis is a great way to define the scope of your UX research project clearly. It helps keep research on track by providing a single statement to come back to and anchor your research in.

Whether you run usability tests or user interviews to assess your hypothesis—Maze's suite of advanced research methods enables you to get the in-depth user and customer insights you need.

Frequently asked questions about research hypothesis

What is the difference between a hypothesis and a problem statement in UX?

A research hypothesis describes the prediction or method of solving that problem. A problem statement, on the other hand, identifies a specific issue in your design that you intend to solve. A problem statement will typically include a user persona, an issue they have, and a desired outcome they need.

How many hypotheses should a UX research problem have?

Technically, there are no limits to the amount of hypotheses you can have for a certain problem or study. However, you should limit it to one hypothesis per specific issue in UX research. This ensures that you can conduct focused testing and reach clear, actionable results.

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Understanding Science

How science REALLY works...

• Understanding Science 101
• Misconceptions
• Testing ideas with evidence is at the heart of the process of science.
• Scientific testing involves figuring out what we would  expect  to observe if an idea were correct and comparing that expectation to what we  actually  observe.

Misconception:  Science proves ideas.

Misconception:  Science can only disprove ideas.

Correction:  Science neither proves nor disproves. It accepts or rejects ideas based on supporting and refuting evidence, but may revise those conclusions if warranted by new evidence or perspectives.  Read more about it.

Testing scientific ideas

Testing ideas about childbed fever.

As a simple example of how scientific testing works, consider the case of Ignaz Semmelweis, who worked as a doctor on a maternity ward in the 1800s. In his ward, an unusually high percentage of new mothers died of what was then called childbed fever. Semmelweis considered many possible explanations for this high death rate. Two of the many ideas that he considered were (1) that the fever was caused by mothers giving birth lying on their backs (as opposed to on their sides) and (2) that the fever was caused by doctors’ unclean hands (the doctors often performed autopsies immediately before examining women in labor). He tested these ideas by considering what expectations each idea generated. If it were true that childbed fever were caused by giving birth on one’s back, then changing procedures so that women labored on their sides should lead to lower rates of childbed fever. Semmelweis tried changing the position of labor, but the incidence of fever did not decrease; the actual observations did not match the expected results. If, however, childbed fever were caused by doctors’ unclean hands, having doctors wash their hands thoroughly with a strong disinfecting agent before attending to women in labor should lead to lower rates of childbed fever. When Semmelweis tried this, rates of fever plummeted; the actual observations matched the expected results, supporting the second explanation.

Testing in the tropics

Let’s take a look at another, very different, example of scientific testing: investigating the origins of coral atolls in the tropics. Consider the atoll Eniwetok (Anewetak) in the Marshall Islands — an oceanic ring of exposed coral surrounding a central lagoon. From the 1800s up until today, scientists have been trying to learn what supports atoll structures beneath the water’s surface and exactly how atolls form. Coral only grows near the surface of the ocean where light penetrates, so Eniwetok could have formed in several ways:

Hypothesis 2: The coral that makes up Eniwetok might have grown in a ring atop an underwater mountain already near the surface. The key to this hypothesis is the idea that underwater mountains don’t sink; instead the remains of dead sea animals (shells, etc.) accumulate on underwater mountains, potentially assisted by tectonic uplifting. Eventually, the top of the mountain/debris pile would reach the depth at which coral grow, and the atoll would form.

Which is a better explanation for Eniwetok? Did the atoll grow atop a sinking volcano, forming an underwater coral tower, or was the mountain instead built up until it neared the surface where coral were eventually able to grow? Which of these explanations is best supported by the evidence? We can’t perform an experiment to find out. Instead, we must figure out what expectations each hypothesis generates, and then collect data from the world to see whether our observations are a better match with one of the two ideas.

If Eniwetok grew atop an underwater mountain, then we would expect the atoll to be made up of a relatively thin layer of coral on top of limestone or basalt. But if it grew upwards around a subsiding island, then we would expect the atoll to be made up of many hundreds of feet of coral on top of volcanic rock. When geologists drilled into Eniwetok in 1951 as part of a survey preparing for nuclear weapons tests, the drill bored through more than 4000 feet (1219 meters) of coral before hitting volcanic basalt! The actual observation contradicted the underwater mountain explanation and matched the subsiding island explanation, supporting that idea. Of course, many other lines of evidence also shed light on the origins of coral atolls, but the surprising depth of coral on Eniwetok was particularly convincing to many geologists.

• Take a sidetrip

Visit the NOAA website to see an animation of coral atoll formation according to Hypothesis 1.

• Teaching resources

Scientists test hypotheses and theories. They are both scientific explanations for what we observe in the natural world, but theories deal with a much wider range of phenomena than do hypotheses. To learn more about the differences between hypotheses and theories, jump ahead to  Science at multiple levels .

• Use our  web interactive  to help students document and reflect on the process of science.
• Learn strategies for building lessons and activities around the Science Flowchart: Grades 3-5 Grades 6-8 Grades 9-12 Grades 13-16
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How to Write a Hypothesis? [Tips with Examples]

Click here if you have ever found yourself in the position of having to wrestle with the development of a hypothesis for your research paper. As an expert writer, I have seen that this is where most students begin to sweat. It is a potpourri of theory and practice, hence rather intimidating. But not to worry because I have got your back. This guide is a pool of tips and tricks for writing a hypothesis to set the stage for compelling research.

What is a Hypothesis?

A hypothesis is a tentative statement, usually in the form of an educated guess, that provides a probable explanation for something either a phenomenon or a relationship between variables. This will, therefore, form a basis for conducting experiments and research studies, hence laying down the course of your investigation and mainly laying the ground for your conclusion.

A good hypothesis should be:

Specific and clear

Testable and falsifiable

Based upon existing knowledge

Logically consistent

Types of Hypothesis

There are different kinds of hypotheses used in research, all of which serve different purposes depending on the nature of the study. Here are eight common types:

1. The null hypothesis (H0):  asserts that there is no effect or relationship between variables. This forms a baseline for comparison. Example: "There is no difference in test scores for students who study music and for those who do not."

2. Alternative Hypothesis (H1): The hypothesis that postulates some effect or relationship between variables; it is, therefore, the opposite of the null hypothesis. For instance, "Students who study with music have different test scores than those who study in silence."

3. Simple Hypothesis: The hypothesis that states a relationship between two variables: one independent and one dependent. For example, "More sunlight increases plant growth."

4. Complex Hypothesis: This hypothesis involves the relationship of more than one variable. For example, "More sunlight and water increase plant growth."

5. Directional Hypothesis: The hypothesis which specifies the direction of the effect between variables. For instance, "Students who study with music will have higher test scores than students who study in silence."

6. Non-Directional Hypothesis: This is a hypothesis used where the relationship is indicated, but the direction is not specified. For example, "There is a difference in test scores between students who study with music and those who study in silence."

7. Associative Hypothesis: This hypothesis merely states that the change in one variable is associated with a change in another. It does not indicate cause and effect. For example: "There is a relationship between study habits and academic performance."

8. Causal Hypothesis: This hypothesis states that one variable causes a change in another. For example: "Increased study time results in higher test scores."

Understanding such types of hypotheses will help in the selection of the correct hypothesis for your research and in making your analysis clear and effective.

5 Steps to Write a Good Hypothesis [With Examples]

An excellent hypothesis provides a backbone to any scientific research. Leave some help behind in writing one? Follow this easy guide:

Step 1: Ask a Question

First, you must understand what your research question is. Suppose you want to carry out an experiment on plant growth. Your question can be, "How does sunlight affect plant growth?"

Use WPS AI to help when you get stuck. Feed it a topic, and it will come up with related questions to ask.

Step 2: Do Preliminary Research

Do some research to see what's already known about your topic. That way, you can build upon existing knowledge.

Research information in journals, books and credible websites. Then summarize what you read. This will help you formulate your hypothesis.

Step 3: Define Variables

Identify your variables:

Independent Variable: What you manipulate. For example, the amount of sun.

Dependent Variable: What you measure. For example, plant growth rate.

Clearly defining these makes your hypothesis specific and testable.

Step 4: State Your Hypothesis

State your question in the form of a hypothesis. Here are some examples:

If  then: "If plants receive more sunlight, then they will grow faster."

Comparative statements: "Plants receiving more sunlight grow faster than plants receiving less."

Correlation statements: "There is positive correlation between sunlight and plant growth." This kind of pattern makes your hypothesis easy to test.

Step 5: Refine Your Hypothesis

Revise your hypothesis to be clear and specific, and elicit feedback to improve it.

You will also need a null hypothesis, which says that there is no effect or relationship between variables. An example would be, "Sunlight has no effect on the growth of plants."

With these steps, you are now bound to come up with a testable hypothesis. WPS AI can help you in this process more efficiently.

Characteristics of a Good Hypothesis

A good hypothesis is seen as the backbone of doing effective research. Following are some key characteristics that define a good hypothesis:

A good hypothesis has to be testable either by experimentation or observation. The hypothesis should clearly predict what can be measured or observed. For example, "If it receives more sunlight, the plant will grow taller" is a testable hypothesis since it states what can be measured.

Falsifiable

A hypothesis has to be falsifiable: it should be able to prove it wrong. This feature is important because it accommodates testing in science. For example, the statement "All swans are white" is falsifiable since it just takes one black swan to disprove the claim.

A good hypothesis should be grounded in current knowledge and should be properly reasoned. It should be broad or reasonable within existing knowledge. For example, "Increasing the amount of sunlight will boost plant growth" makes sense, in that it tallies with generally known facts about photosynthesis.

Specific and Clear

What is needed is clarity and specificity. A hypothesis has to be brief, yet free from ambiguity. For instance, "Increased sunlight leads to taller plants" is clear and specific whereas "Sunlight affects plants" is too vague.

Built upon Prior Knowledge

A good hypothesis is informed by prior research and existing theories. The available knowledge enlightens it to build on what is known to find new relationships or effects. For example, "Given photosynthesis requires sunlight, increasing sunlight will enhance plant growth" is informed by available scientific understanding.

Ethical Considerations

Finally, a good hypothesis needs to consider the ethics involved. The research should not bring damage to participants or the environment. For instance, "How the new drug will affect a human when tested without testing it on animals" may present an ethical concern.

Checklist for Reviewing Your Hypothesis

To be certain that your hypothesis has the following characteristics, use this checklist to review your hypothesis:

1. Is the hypothesis testable through experimentation or observation?

2. Can the hypothesis be proven false?

3. Is the hypothesis logically deduced from known facts?

4. Is your hypothesis clear and specific?

5. Does your hypothesis relate to previous research or theories?

6. Will there be any ethical issues with the proposed research?

7. Are your independent and dependent variables well defined?

8. Is your hypothesis concise and ambiguity free?

9. Did you get feedback to help in refining your hypothesis?

10. Does your hypothesis contain a null hypothesis for comparison?

By making sure that your hypothesis has these qualities, you are much more likely to set yourself on the course of higher-quality research and larger impacts. WPS AI can help fine-tune a hypothesis to ensure it is well-structured and clear.

Using WPS to Perfect your Hypothesis

Drafting a good hypothesis is the real inception of any research project. WPS AI, with its advanced language functions, can very strongly improve this stage of your study. Here's how WPS AI can help you perfect your hypothesis:

Check Grammar and Syntax

Grammar and punctuation errors can make your hypothesis weak. WPS AI checks and corrects this with the assurance that your hypothesis is as clear as possible and professional in its presentation. For example, when your hypothesis is written, "If the temperature increases then plant growth will increases", WPS AI can correct it to "If the temperature increases, then plant growth will increase."

Rewrite Your Hypothesis for Clarity

There needs to be a clear hypothesis. WPS AI can suggest ways to reword your hypothesis so that it makes sense. If your original hypothesis is, "More sunlight will result in more significant plant growth due to photosynthesis," WPS AI can suggest, "Increased sunlight will lead to greater plant growth through enhanced photosynthesis."

Automatic Content Expansion

Sometimes, your hypothesis or the related paragraphs may require more detail. WPS AI's [Continue Writing] feature can help enlarge the content. For example, after having written, "This study will examine the effects of sunlight on plant growth", using [Continue Writing] it can enlarge it to, "This research paper is going to study how sunlight affects the growth of plants by measuring their height and their health under different amounts of sunlight over a period of six weeks."

WPS AI is a great tool that can help you in drafting a good hypothesis for your research. It will help you check grammar, syntax, clarity, and completeness. Using WPS AI , you will be assured that the results of your hypothesis will be well-written and clear to understand.

What is the difference between a hypothesis and a theory?

The hypothesis is one single testable prediction regarding some phenomenon. The theory is an explanation for some part of the natural world which is well-substantiated by a body of evidence, together with multiple hypotheses.

What do I do if my hypothesis isn't supported by my data?

If your results turn out not to support your hypothesis, analyze the data again to see why your result rejects your hypothesis. Do not manipulate the observations or experiment so that it leads to your hypothesis.

Can there be more than one hypothesis in a research study?

Yes, there may be more than one hypothesis, especially when one research study is examining several interrelated phenomena or variables. Each hypothesis has to be separately and clearly stated and tested.

Correct formulation of a strong, testable hypothesis is one of the most critical steps in the application of the scientific method and within academic research. The steps provided in this article will help you write a hypothesis that is clear, specific, and based on available knowledge. Give the tools and tips a try to elevate your academic writing and kick your research up a notch.

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Title: hypothesis testing for general network models.

Abstract: The network data has attracted considerable attention in modern statistics. In research on complex network data, one key issue is finding its underlying connection structure given a network sample. The methods that have been proposed in literature usually assume that the underlying structure is a known model. In practice, however, the true model is usually unknown, and network learning procedures based on these methods may suffer from model misspecification. To handle this issue, based on the random matrix theory, we first give a spectral property of the normalized adjacency matrix under a mild condition. Further, we establish a general goodness-of-fit test procedure for the unweight and undirected network. We prove that the null distribution of the proposed statistic converges in distribution to the standard normal distribution. Theoretically, this testing procedure is suitable for nearly all popular network models, such as stochastic block models, and latent space models. Further, we apply the proposed method to the degree-corrected mixed membership model and give a sequential estimator of the number of communities. Both simulation studies and real-world data examples indicate that the proposed method works well.

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• Null and Alternative Hypotheses | Definitions & Examples

Null & Alternative Hypotheses | Definitions, Templates & Examples

Published on May 6, 2022 by Shaun Turney . Revised on June 22, 2023.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

• Null hypothesis ( H 0 ): There’s no effect in the population .
• Alternative hypothesis ( H a or H 1 ) : There’s an effect in the population.

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, similarities and differences between null and alternative hypotheses, how to write null and alternative hypotheses, other interesting articles, frequently asked questions.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”:

• The null hypothesis ( H 0 ) answers “No, there’s no effect in the population.”
• The alternative hypothesis ( H a ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample. It’s critical for your research to write strong hypotheses .

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

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The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept . Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect,” “no difference,” or “no relationship.” When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

You can never know with complete certainty whether there is an effect in the population. Some percentage of the time, your inference about the population will be incorrect. When you incorrectly reject the null hypothesis, it’s called a type I error . When you incorrectly fail to reject it, it’s a type II error.

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis ( H a ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect,” “a difference,” or “a relationship.” When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes < or >). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

• They’re both answers to the research question.
• They both make claims about the population.
• They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

General template sentences

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

• Null hypothesis ( H 0 ): Independent variable does not affect dependent variable.
• Alternative hypothesis ( H a ): Independent variable affects dependent variable.

Test-specific template sentences

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (“ x affects y because …”).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses . In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Antibodies From Long COVID Patients Provide Clues to Autoimmunity Hypothesis

BY ISABELLA BACKMAN August 5, 2024

Promising new research supports that autoimmunity—in which the immune system targets its own body—may contribute to Long COVID symptoms in some patients.

As covered previously in this blog, researchers have several hypotheses to explain what causes Long COVID, including lingering viral remnants, the reactivation of latent viruses, tissue damage, and autoimmunity.

Now, in a recent study , when researchers gave healthy mice antibodies from patients with Long COVID, some of the animals began showing Long COVID symptoms—specifically heightened pain sensitivity and dizziness. It is among the first studies to offer enticing evidence for the autoimmunity hypothesis. The research was led by Akiko Iwasaki, PhD , Sterling Professor of Immunobiology at Yale School of Medicine (YSM).

“We believe this is a big step forward in trying to understand and provide treatment to patients with this subset of Long COVID,” Iwasaki said.

Iwasaki zeroed in on autoimmunity in this study for several reasons. First, Long COVID’s persistent nature suggested that a chronic triggering of the immune system might be at play. Second, women between ages 30 and 50, who are most susceptible to autoimmune diseases, are also at a heightened risk for Long COVID. Finally, some of Iwasaki’s previous research had detected heightened levels of antibodies in people infected with SARS-CoV-2.

Mice given antibodies show signs of Long COVID symptoms

Iwasaki’s team isolated antibodies from blood samples obtained from the Mount Sinai-Yale Long COVID study . They transferred these antibodies into mice and then conducted multiple experiments designed to look for changes in behavior that may indicate the presence of specific symptoms. For many of these experiments, mice that received antibodies [the experimental group] behaved no differently than mice that had not [the control group].

However, a few experiments revealed striking changes in the behavior of the experimental mice. These included:

• Pain sensitivity test: Some experimental mice were quicker to react after being placed on a heated plate.
• Coordination and balance test: Some experimental mice struggled to balance on a rotarod (rotating rod) compared to control mice.
• Grip strength test: Some of the experimental mice applied less force with their paws.

Among the mice that showed behavioral changes, the researchers identified which patients their antibodies came from and what symptoms they had experienced. Interestingly, of the mice that showed heightened pain, 85% received antibodies from patients that reported pain as one of their Long COVID symptoms. Additionally, 89% of mice that had demonstrated loss of balance and coordination on the rotarod test had received antibodies from patients who reported dizziness. Furthermore, 91% of mice that showed reduced strength and muscle weakness received antibodies from patients who reported headache and 55% from patients who reported tinnitus. More research is needed to better understand this correlation.

The autoimmunity hypothesis has recently been further supported by a research group in the Netherlands led by Jeroen den Dunnen, DRS , associate professor at Amsterdam University Medical Center, which also found a link between patients’ Long COVID antibodies and corresponding symptoms in mice.

Treatments for autoimmunity may help some Long COVID patients

Diagnosing and treating Long COVID requires doctors to understand what causes the disease. The new study suggests that treatments targeting autoimmunity, such as B cell depletion therapy or plasmapheresis, might alleviate symptoms in some patients by removing the disease-causing antibodies.

Intravenous immunoglobulin (IVIg) is another therapy used for treating autoimmune diseases like lupus in which patients receive antibodies from healthy donors. While its exact mechanism is still unclear, the treatment can help modulate the immune system and reduce inflammation. Could this treatment help cases of Long COVID that are caused by autoimmunity?

A 2024 study led by Lindsey McAlpine, MD , instructor at YSM and first author, and Serena Spudich, MD , Gilbert H. Glaser Professor of Neurology at YSM and principal investigator, found that IVIg might help improve small fiber neuropathy—a condition associated with numbness or painful sensations in the hands and feet—caused by Long COVID. Iwasaki is hopeful that future clinical trials might reveal the benefits of this treatment in helping some of the other painful symptoms of the diseases.

Other drugs are also in the pipeline, such as FcRn inhibitors. FcRn is a receptor that binds to antibodies and recycles them. Blocking this receptor could help bring down levels of circulating antibodies in the blood. An FcRn receptor was recently approved by the FDA for treating myasthenia gravis, another kind of autoimmune disease.

The study could also help researchers create diagnostic tools for evaluating which patients have Long COVID induced by autoimmunity so that doctors can identify who is most likely to benefit from treatments such as these.

Iwasaki plans to continue researching why and how autoantibodies might cause Long COVID, as well as conduct randomized clinical trials on promising treatments. She is also conducting similar antibody transfer studies in other post-acute infection syndromes, such as myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS).

In the meantime, she is excited about her team’s promising results. “Seeing this one-to-one correlation of antibodies that cause pain from patients who reported pain is really gratifying to me as it suggests a causal link,” she says. “It’s a first step, but I think it’s a big one.”

Isabella Backman is associate editor and writer at Yale School of Medicine.

The last word by Lisa Sanders, MD:

I am very excited by this research, which suggests that at least some of the symptoms of Long COVID are driven by autoimmunity. If so, then this suggests that there may be a way to test for some versions of Long COVID. And if we could identify the patients who have an autoimmune-driven disease, we have treatments to try that have been used with success in other autoimmune diseases. Many of the autoimmune diseases are treated with medications that suppress the immune system. These are powerful medicines that can leave an individual at risk for infection, so they must be thoughtfully applied to patients with evidence of immune system involvement.

I feel as though every blog post here ends with the possibility of better testing and better treatment, but what makes this different is that it points in a very specific direction and leads to the kind of specific questions that help get to useful answers. Which antibodies are involved? Which cells? And finally, can we develop treatments that are specific to those antibodies or to their targets? These are exciting questions, which will, I hope, lead to useful answers.

Read other installments of Long COVID Dispatches here .

If you’d like to share your experience with Long COVID for possible use in a future post (under a pseudonym), write to us at: [email protected]

Information provided in Yale Medicine content is for general informational purposes only. It should never be used as a substitute for medical advice from your doctor or other qualified clinician. Always seek the individual advice of your health care provider for any questions you have regarding a medical condition.

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Vesiculation pathways in clathrin-mediated endocytosis

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During clathrin-mediated endocytosis, a patch of flat plasma membrane is internalized to form a vesicle. In mammalian cells, how the clathrin coat deforms the membrane into a vesicle remains unclear and two main hypotheses have been debated. The 'constant area' hypothesis assumes that clathrin molecules initially form a flat lattice on the membrane and deform the membrane by changing its intrinsic curvature while keeping the coating area constant. The alternative 'constant curvature' hypothesis assumes that the intrinsic curvature of the clathrin lattice remains constant during the formation of a vesicle while the surface area it covers increases. Previous experimental studies were unable to unambiguously determine which hypothesis is correct. In this paper, we show that these two hypotheses are only two extreme cases of a continuum of vesiculation pathways if we account for the free energies associated with clathrin assembly and curvature generation. By tracing the negative gradient of the free energy, we define vesiculation pathways in the phase space of the coating area and the intrinsic curvature of clathrin coat. Our results show that, overall, the differences in measurable membrane morphologies between the different models are not as big as expected, and the main differences are most salient at the early stage of endocytosis. Furthermore, the best fitting pathway to experimental data is not compatible with the constant-curvature model and resembles to a constant-area-like pathway where the coating area initially expands with minor changes in the intrinsic curvature, later followed by a dramatic increase in the intrinsic curvature and minor change in the coating area. Our results also suggest that experimental measurement of the tip radius and the projected area of the clathrin coat will be the key to distinguish between models.

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The authors have declared no competing interest.

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